AB316321

重组Anti-JMJD1C抗体[EPR27248-29] - BSA and Azide free

Anti-JMJD1C antibody [EPR27248-29] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal JMJD1C antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

查看别名

Jhdm2c, Kiaa1380, Vglut3, Slc17a8, Probable JmjC domain-containing histone demethylation protein 2C, Jumonji domain-containing protein 1C

2 Images

Supplier Data

Western blot - Anti-JMJD1C antibody [EPR27248-29] - BSA and Azide free (AB316321)

This data was developed using ab316320, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle, colon (PMID : 17353003).

The identity of the bands between 100 kDa and 230 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1 : 180 seconds; Lanes 2-9 : 81 seconds.

All lanes:

Western blot - Anti-JMJD1C antibody [EPR27248-29] (<a href='/products/primary-antibodies/jmjd1c-antibody-epr27248-29-ab316320'>ab316320</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse colon tissue lysate at 20 µg

Lane 6:

Mouse forebrain tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

HL-1 (mouse atrial muscle cell) whole cell lysate at 20 µg

Lane 9:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 261 kDa,124 kDa

false

Supplier Data

Western blot - Anti-JMJD1C antibody [EPR27248-29] - BSA and Azide free (AB316321)

This data was developed using ab316320, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle, colon (PMID : 17353003).

The identity of the lower MW band at approximately 160 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-JMJD1C antibody [EPR27248-29] (<a href='/products/primary-antibodies/jmjd1c-antibody-epr27248-29-ab316320'>ab316320</a>) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 261 kDa,124 kDa

false

Exposure time: 37s

不同偶联物与剂型 (1)

Unconjugated

Anti-JMJD1C antibody [EPR27248-29]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR27248-29

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab316321 is the carrirer-free version of ab316320.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

JMJD1C also known as KDM3C is a member of the Jumonji domain-containing proteins family. It acts as a histone demethylase specifically targeting histone H3 at lysine 9 (H3K9). This enzymatic function plays a role in epigenetic regulation by removing methyl groups from histone proteins influencing chromatin structure and gene expression. The protein has an approximate molecular mass of 170 kDa. It is expressed in various tissues with significant levels found in the testis brain and other central nervous system components.

Biological function summary

JMJD1C regulates gene expression linked to spermatogenesis metabolic processes and neurodevelopmental signaling. It interacts with components of transcriptional complexes having a role in the demethylation of histones to facilitate transcription. The protein also controls hormonal responses by influencing androgen receptor signaling pathways which is important in reproductive tissue function and development.

Pathways

JMJD1C integrates into metabolic and developmental regulatory networks. It is actively involved in the androgen receptor signaling pathway and the Wnt signaling pathway. Its interactions with proteins such as AR (androgen receptor) and components of the Wnt signaling orchestrate gene transcription impacting cellular growth and differentiation. By modulating these pathways JMJD1C links epigenetic modifications to broader physiological processes.

JMJD1C has been associated with cancer and neuropsychiatric disorders. Aberrant expression or mutation of JMJD1C can contribute to prostate cancer given its role in androgen receptor pathways and it has also been implicated in schizophrenia where it may influence neuronal gene expression. The protein's interaction with the androgen receptor and other transcription factors highlights its significance in these pathologies suggesting potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes (By similarity).

See full target information Jmjd1c

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重组Anti-JMJD1C抗体[EPR27248-29] - BSA and Azide free