Anti-JLP抗体(ab12331)
Key features and details
- Rabbit polyclonal to JLP
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-JLP抗体
参阅全部 JLP 一抗 -
描述
兔多克隆抗体to JLP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide within Human JLP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60271 -
阳性对照
- IP: HeLa nuclear extract. WB: HeLa, 293T,Jurkat, 3T3 cell lysates IHC-P: Human testis, and seminoma tissues
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody is affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa.
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| IP |
Use a concentration of 2 - 5 µg/ml.
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| 说明 |
|---|
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. |
|
IP
Use a concentration of 2 - 5 µg/ml. |
靶标
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相关性
JLP is a member of the JNK-interacting protein (JIP) group of scaffold proteins which selectively mediate JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. -
细胞定位
Cytoplasm. Cytoplasm, perinuclear region. Note: Perinuclear distribution in response to stress signals such as UV radiation. -
数据库链接
- Entrez Gene: 9043 Human
- Entrez Gene: 70834 Mouse
- Omim: 605430 Human
- SwissProt: O60271 Human
- SwissProt: Q58A65 Mouse
- SwissProt: Q8CJC2 Mouse
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别名
- C jun amino terminal kinase interacting protein 4 antibody
- c Jun NH2 terminal kinase associated leucine zipper protein antibody
- FLJ13450 antibody
see all
图片
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Western blot - Anti-JLP antibody (ab12331)All lanes : Anti-JLP antibody (ab12331) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 156.8 kDa -
Immunoprecipitation - Anti-JLP antibody (ab12331)Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). ab12331 was used for immunoprecipitation at 3 µg/mg lysate and used for western blot at 1 µg/ml. Lanes 1 and 2 - ab12331 IP, Lane 3 - control IgG.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12331)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human seminoma using ab12331 at 1/1000 with and HRP- conjugated goat anti-rabbit secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab12331 被引用在 22 文献中.
- Tian M et al. JLP/Foxk1/N-cadherin axis fosters a partial epithelial-mesenchymal transition state in epithelial tubular cells. iScience 26:106396 (2023). PubMed: 37013185
- Tian M et al. Loss of JNK-Associated Leucine Zipper Protein Promotes Peritoneal Dialysis-Related Peritoneal Fibrosis. Kidney Dis (Basel) 8:168-179 (2022). PubMed: 35527988
- Okumura F et al. ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation. Sci Rep 11:20880 (2021). PubMed: 34686700
- Yan Q et al. A negative feedback loop between JNK-associated leucine zipper protein and TGF-ß1 regulates kidney fibrosis. Commun Biol 3:288 (2020). PubMed: 32504044
- Yan P et al. LncRNA NEAT1 enhances the resistance of anaplastic thyroid carcinoma cells to cisplatin by sponging miR-9-5p and regulating SPAG9 expression. Int J Oncol 55:988-1002 (2019). PubMed: 31485599