为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-JIP3抗体(ab4545)

  • Datasheet
Reviews (1)Q&A (3)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JIP3 antibody (ab4545)

    Key features and details

    • Goat polyclonal to JIP3
    • Suitable for: IHC-P
    • Reacts with: Mouse
    • Isotype: IgG

    你可能会感兴趣

    蛋白
    Product image
    Recombinant Human IKB alpha protein (ab113133)
    一抗
    Product image
    Anti-IKK beta antibody [EPR6043] (ab124957)
    二抗
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

    查看更多相关产品

    概述

    • 产品名称

      Anti-JIP3抗体
      参阅全部 JIP3 一抗
    • 描述

      山羊多克隆抗体to JIP3
    • 宿主

      Goat
    • 特异性

      This antibody is expected to recognise both human isoforms of this protein.
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Mouse
      预测可用于: Rat, Cow, Human
    • 免疫原

      Synthetic peptide:

      MEIQMDEGGGVV

      , corresponding to N terminal amino acids 2-13 of Human JIP3.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      GenBank Accession Number – NP_055948; NP_203750. LocusLink ID - 23162 (human); 30957 (mouse). Gene Ontology terms - vesicle-mediated transport; kinesin complex; cytoplasm; kinesin binding; MAP-kinase scaffold activity; protein kinase binding; regulation of JNK cascade.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.3
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4545于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins.
    • 序列相似性

      Belongs to the JIP scaffold family.
    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位

      Cytoplasm.
    • Target information above from: UniProt accession Q9UPT6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 537598 Cow
      • Entrez Gene: 23162 Human
      • Entrez Gene: 30957 Mouse
      • Entrez Gene: 302983 Rat
      • Omim: 605431 Human
      • SwissProt: Q9UPT6 Human
      • SwissProt: Q9ESN9 Mouse
      • Unigene: 207763 Human
      • Unigene: 43081 Mouse
      see all
    • 别名

      • C-jun-amino-terminal kinase-interacting protein 3 antibody
      • FLJ00027 antibody
      • Homolog of Drosophila Sunday driver 2 antibody
      • JIP-3 antibody
      • JIP3 antibody
      • JIP3_HUMAN antibody
      • JNK / Stress Activated Protein Kinase Associated Protein 1 antibody
      • JNK Interacting Protein 3 antibody
      • JNK MAP kinase scaffold protein 3 antibody
      • JNK-interacting protein 3 antibody
      • JNK/SAPK associated protein 1 antibody
      • JSAP1 antibody
      • KIAA1066 antibody
      • MAPK8IP3 antibody
      • Mitogen Activated Protein kinase 8 Interacting Protein 3 antibody
      • Mitogen-activated protein kinase 8-interacting protein 3 antibody
      • Syd antibody
      • SYD2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JIP3 antibody (ab4545)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JIP3 antibody (ab4545)
      ab4545 (4µg/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Shows axonal staining in various regions

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab4545?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4545 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-JIP3 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (heart)
    Loading amount
    60 µg
    Specification
    heart
    Gel Running Conditions
    Non-reduced Denaturing (7.5)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 15 2012

    Question

    Hi Jennifer, I am wondering that Fright/packing fee $60 is USD or CaD? This price show in website. I am a little confused.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2004

    Answer

    Thank you for your email. To ship to Canada, the cost is 60 USD (United States Dollar). If you have any more questions, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Dec 14 2004

    Question

    Thank you very much for your information I want to oder Jip3 antibody.It is impossible for me to get the award $300 of Conference Travel Money to the first researcher who sends you positive feedback. I am just wondering if I get any discount of this purchase?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2004

    Answer

    Thank you for your email. We have not yet received feedback regarding this antibody so it is still possible for you to obtain the $300 Conference Travel Award. A discount is not available for this antibody. If you have any more questions, just let us know.

    Read More

    Abcam Scientific Support

    回复于 Dec 13 2004

    Question

    I am a posdoctor in department of Biochemistry and Medical Genetics o the university of Manitoba at Canada. I want t order JIP3 antibody. I have never used this antibody before. So, Can you send me some detail information about this antibody? For examples, I would like to some picture of western blot of this antibody . If I order this antibody tomorrow, when will I get it?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2004

    Answer

    Thank you for your enquiry. Ab4545 is a Fast-Track antibody; Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. More details regarding the Fast-Track antibody terms of use can be found on the online datasheet for ab4545. Ab4545 has been tested in peptide ELISA, antibody detection limit at a dilution of 1/4000. In Western blotting, no signal has been obtained yet, but low background was observed in Human Heart lysate at up to 2µg/ml. We don't have an image available at this time or any additional information. If you choose to purchase this antibody, we would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. Ab4545 is currently out of stock; if you placed an order tomorrow, you would get it in approximately 2-3 weeks time. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Dec 07 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有