Anti-JDP2抗体(ab40916)
Key features and details
- Rabbit polyclonal to JDP2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-JDP2抗体 -
描述
兔多克隆抗体to JDP2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Rat
不与反应:
Mouse -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human JDP2.
参阅Abcam的专有抗源政策 (Peptide available as ab41625.) -
阳性对照
- ab40916 gave a positive signal in WB on the following lysates: HeLa whole cell, HeLa nuclear, Jurkat whole cell, Jurkat nuclear, HepG2 nuclear, Du145 whole cell and LnCAP nuclear. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
翻译后修饰
Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 122953 Human
- Entrez Gene: 116674 Rat
- Omim: 608657 Human
- SwissProt: Q8WYK2 Human
- SwissProt: Q78E65 Rat
- Unigene: 196482 Human
- Unigene: 10721 Rat
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别名
- Jdp2 antibody
- JDP2_HUMAN antibody
- Jun dimerization protein 2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-JDP2 antibody (ab40916)
ab40916 staining JDP2 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab40916 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-JDP2 antibody (ab40916)All lanes : Anti-JDP2 antibody (ab40916) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lane 5 :Hep G2 nuclear extract lysate (ab14660)
Lane 6 : Du145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 7 : LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab40916 被引用在 5 文献中.
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Kong X et al. Defining UHRF1 Domains that Support Maintenance of Human Colon Cancer DNA Methylation and Oncogenic Properties. Cancer Cell 35:633-648.e7 (2019). PubMed: 30956060
- Mansour MR et al. JDP2: An oncogenic bZIP transcription factor in T cell acute lymphoblastic leukemia. J Exp Med 215:1929-1945 (2018). PubMed: 29941549
- Tanigawa S et al. Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis. Cell Death Dis 4:e921 (2013). WB ; Human . PubMed: 24232097
- Chiou SS et al. Control of Oxidative Stress and Generation of Induced Pluripotent Stem Cell-like Cells by Jun Dimerization Protein 2. Cancers (Basel) 5:959-84 (2013). PubMed: 24202329