Anti-Japanese encephalitis virus NS1 glycoprotein抗体[MV12/2/A5-1/6] (ab41651)
![Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)](https://www.abcam.cn/ps/products/41/ab41651/Images/ab41651-55009-anti-japanese-encephalitis-virus-ns1-glycoprotein-antibody-jn1-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)")
Key features and details
- Mouse monoclonal [MV12/2/A5-1/6] to Japanese encephalitis virus NS1 glycoprotein
- Suitable for: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISA
- Reacts with: Japanese encephalitis virus
- Isotype: IgG3
概述
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产品名称
Anti-Japanese encephalitis virus NS1 glycoprotein抗体[MV12/2/A5-1/6]
参阅全部 Japanese encephalitis virus NS1 glycoprotein 一抗 -
描述
小鼠单克隆抗体[MV12/2/A5-1/6] to Japanese encephalitis virus NS1 glycoprotein -
宿主
Mouse -
经测试应用
适用于: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISAmore details -
种属反应性
与反应: Japanese encephalitis virus -
免疫原
Full length native protein (purified) corresponding to Japanese encephalitis virus Japanese encephalitis virus NS1 glycoprotein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Proclin 150
Constituents: 10% BSA, 89.9% RPMI 1640 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
MV12/2/A5-1/6 -
同种型
IgG3 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41651于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19635909
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 20581148
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
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| WB |
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
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| ICC/IF |
1/5 - 1/20.
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| ELISA |
Use at an assay dependent concentration.
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| 说明 |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 19635909 |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 20581148 ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody. |
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WB
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa. |
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ICC/IF
1/5 - 1/20. |
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ELISA
Use at an assay dependent concentration. |
靶标
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相关性
The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular. -
数据库链接
- SwissProt: P27395 Japanese encephalitis virus
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别名
- Japanese encephalitis non-structural protein 1 antibody
- Japanese encephalitis NS1 antibody
- Japanese encephalitis virus non-structural protein 1 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
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![Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)](https://www.abcam.cn/ps/products/41/ab41651/Images/ab41651-55008-anti-japanese-encephalitis-virus-ns1-glycoprotein-antibody-jn1-western-blot.jpg)
Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)
Predicted band size: 46 kDa
Observed band size: 46,92 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible cleavage fragment), 50 kDa (possible cleavage fragment)
This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab41651 被引用在 9 文献中.
- Sarkar R et al. Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets. J Gen Virol 102:N/A (2021). PubMed: 33095129
- Li XF et al. Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nat Commun 9:673 (2018). PubMed: 29445153
- Sharma N et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep 6:27685 (2016). WB ; Japanese encephalitis virus . PubMed: 27282499
- Sharma N et al. miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflammation 12:30 (2015). WB ; Japanese encephalitis virus . PubMed: 25889446
- Sharma M et al. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Autophagy 10:1637-51 (2014). PubMed: 25046112
- Li XF et al. A chimeric dengue virus vaccine using Japanese encephalitis virus vaccine strain SA14-14-2 as backbone is immunogenic and protective against either parental virus in mice and nonhuman primates. J Virol 87:13694-705 (2013). PubMed: 24109223
- Zhang T et al. Anti- Japanese-Encephalitis-Viral Effects of Kaempferol and Daidzin and Their RNA-Binding Characteristics. PLoS One 7:e30259 (2012). WB ; Japanese encephalitis virus . PubMed: 22276167
- Aleyas AG et al. Multifront Assault on Antigen Presentation by Japanese Encephalitis Virus Subverts CD8+ T Cell Responses. J Immunol : (2010). Flow Cyt . PubMed: 20581148
- Aleyas AG et al. Functional modulation of dendritic cells and macrophages by Japanese encephalitis virus through MyD88 adaptor molecule-dependent and -independent pathways. J Immunol 183:2462-74 (2009). IHC-FoFr . PubMed: 19635909