Anti-JAM-C抗体(ab224327)
Key features and details
- Rabbit polyclonal to JAM-C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-JAM-C抗体
参阅全部 JAM-C 一抗 -
描述
兔多克隆抗体to JAM-C -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human JAM-C aa 100-250.
Database link: Q9BX67 -
阳性对照
- WB: BEWO cell lysate. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224327于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Participates in cell-cell adhesion. It is a counterreceptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. -
组织特异性
Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level). -
疾病相关
Defects in JAM3 are the cause of hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC) [MIM:613730]. A syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain tissue, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue. -
序列相似性
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors. -
细胞定位
Cell membrane. Cell junction > desmosome. Secreted > extracellular space. In epithelial cells, it is expressed at desmosomes but not at tight junctions. Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitement of JAM3 to cell-cell contacts. - Information by UniProt
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数据库链接
- Entrez Gene: 83700 Human
- Omim: 606871 Human
- SwissProt: Q9BX67 Human
- Unigene: 150718 Human
- Unigene: 728339 Human
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别名
- FLJ14529 antibody
- JAM 2 antibody
- JAM 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (ab224327)
Paraffin-embedded human fallopian tube stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong positivity in cilia in glandular cells.
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Western blot - Anti-JAM-C antibody (ab224327)Anti-JAM-C antibody (ab224327) at 1/100 dilution + BEWO cell lysate
Developed using the ECL technique.
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (ab224327)Paraffin-embedded human skeletal muscle stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows no cytoplasmic positivity in myocytes as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (ab224327)Paraffin-embedded human testis stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong membranous positivity in cells in seminiferous ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (ab224327)Paraffin-embedded human kidney stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells in tubules.
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Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody (ab224327)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for JAM-C (green) using ab224327 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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