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AB32101

Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) 抗体 [E132]

Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

3

(4 Reviews)

|

(304 Publications)

Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) is a rabbit monoclonal antibody detecting JAK1 in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 200 publications
- Trusted since 2006

查看别名

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2

12 Images
Flow Cytometry (Intracellular) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells starved of serum for 16 hours then treated with 1 mM Pervanadate for 30 minutes labeling JAK2 (phospho Y1007 + Y1008) with ab32101 at 1/20 dilution (10 ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. Unstimulated Jurkat cells were used as a negative control (Green).

Immunocytochemistry/ Immunofluorescence - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP cells labelling JAK2 (phospho Y1007 + Y1008) with ab32101 at a dilution of 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

ab32101 showing positive staining in Human differentiated squamous cell carcinoma of the cervix tissue at 1/10000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as secondary antibody. Antigen retreival was carried out by Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Nuclear staining on human differentiated squamous cell carcinoma of the cervix without alkaline phosphatase treatment (image A). No staining on human differentiated squamous cell carcinoma of the cervix with alkaline phosphatase treatment (image B)

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

Lab

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Exposure time :
Left image : 1 second
Right image : 5 minutes

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) treated with 50mM Pervanadate for 5 minutes whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

Observed band size: 120 kDa,60 kDa

false

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

Lab

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/5000 dilution

Lane 1:

Untreated Jurkat cells whole cell lysates at 10 µg

Lane 2:

Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates at 10 µg

Lane 3:

Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

Observed band size: 120 kDa

false

Exposure time: 5s

Dot Blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • Dot

Unknown

Dot Blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Dot blot analysis of human JAK2 (phospho Y1007 & Y1008) phospho peptide (Lane 1), JAK2 (phospho Y1007) phospho peptide (Lane 2), JAK2 (phospho Y1008) phospho peptide (Lane 3) and JAK2 non-phospho peptide (Lane 4) labelling JAK2 (phospho Y1007 & Y1008) with ab32101 at a dilution of 1/1000.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000.

Blocking/Dilution buffer : 5% NFDM/TBST.

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

AbReview18053****

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/2000 dilution

Lane 1:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 0 hours. at 30 µg

Lane 2:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 15 minutes. at 30 µg

Lane 3:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 30 minutes. at 30 µg

Lane 4:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 1 hour. at 30 µg

Lane 5:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 2 hours. at 30 µg

Lane 6:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 4 hours. at 30 µg

Lane 7:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 6 hours. at 30 µg

Lane 8:

Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 24 hours. at 30 µg

Secondary

All lanes:

An HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 110 kDa,55 kDa

true

This image is courtesy of an anonymous Abreview

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

Supplier Data

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

The extra bands are undefined.

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

Lane 1:

Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

Hepa1-6 (Mouse hepatoma epithelial cell) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

Lane 3:

MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate at 15 µg

Lane 4:

MEF (Mouse embryonic fibroblast (immortalized)) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

false

ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • ELISA

Supplier Data

ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Direct ELISA antigen dose-response curve using ab32101 at 0~1000 ng/mL. Antigen concentration of 10 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500) was used as the secondary antibody.

This antibody preferentially recognizes phospho Y1007. When the concentration of peptides is lower than 10 ng/mL, it cannot recognize phospho Y1008.

ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • ELISA

Supplier Data

ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Direct ELISA antigen dose-response curve using ab32101 at 0~1000 ng/mL. Antigen concentration of 100 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

This antibody preferentially recognizes phospho Y1007. When the concentration of peptides is higher than 100 ng/mL, it also recognizes phospho Y1008.

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

Supplier Data

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

Lane 1:

Mouse hippocampus lysate at 15 µg

Lane 2:

Mouse P240 hippocampus lysate at 15 µg

Lane 3:

Mouse P7 hippocampus lysate at 15 µg

Lane 4:

Rat hippocampus lysate at 15 µg

Lane 5:

Rat P7 hippocampus lysate at 15 µg

Lane 6:

Rat brain cortex lysate at 15 µg

Lane 7:

Human brain lysate at 15 µg

Lane 8:

Mouse brain lysate at 15 µg

Lane 9:

Rat brain lysate at 15 µg

Lane 10:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 11:

C6 (Rat glial tumor glial cell) treated with 50mM pervanadate for 5 minutes whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

false

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)
  • WB

Lab

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

Blocking and dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

Lane 1:

293T cells transfected with a Human JAK1 expression vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human JAK1 expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human JAK1 (mutated Y1034A, mutated Y1035A) expression vector containing a His tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Human JAK1 (mutated Y1034A, mutated Y1035A) expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Human JAK2 expression vector containing a His tag whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a Human JAK2 expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a Human JAK2 (mutated Y1007A, mutated Y1008A) expression vector containing a His tag whole cell lysate at 20 µg

Lane 8:

293T cells transfected with a Human JAK2 (mutated Y1007A, mutated Y1008A) expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa

false

Exposure time: 7s

不同偶联物与剂型 (10)

  • Carrier free

    Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] - BSA & Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 660 APC

    APC Anti-JAK2 (phospho Y1007 + Y1008)+ JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • HRP

    HRP Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 578 PE

    PE Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

E132

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human, Rat

应用

Dot, ELISA, ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody is phospho-specific and only detects phosphorylated JAK2 on Tyrosine 1007 and 1008 (pY1007+Y1008) and JAK1 on Tyrosine 1034 and 1035 (pY1034+Y1035). According to our ELISA results, this antibody preferentially recognizes phospho Y1007. Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of JAK1?
Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) detects a band for JAK1 (UniProt: O60674) at a molecular weight of 130kDa.

Trusted by the scientific community
Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) was first used in a scientific publication in 2006 and has been cited over 200 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.
Biological function summary

JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.

Pathways

The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells' function.

JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed : 15690087, PubMed : 7615558, PubMed : 9657743, PubMed : 15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed : 15690087, PubMed : 9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed : 9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed : 21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed : 20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed : 21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed : 19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed : 25644777).
See full target information JAK2 phospho Y1007 + Y1008

其他靶点

JAK1 phospho Y1034 + Y1035
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文献 (304)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:315 PubMed40859234

2025

AZD5153 enhances the chemo-sensitivity of gemcitabine on pancreatic cancer cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Haixin Zhu,Minmin Shen,Yiqian Zhu,Ruoqi Wang,Rong Dong,Yuyu Huang,Lulin Zhu,Ying Li,Youyou Yan,Jiang Lou,Bo Zhang,Nengming Lin,Biqin Tan

Neoplasia (New York, N.Y.) 68:101219 PubMed40840329

2025

Prkci activates Jak2/Stat3 signaling to promote tumor angiogenesis: Short Name: Prkci in tumor angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Guangshi Liu,Wenbin Zhang,Tao Li,Xinhui Yang

Materials today. Bio 33:102027 PubMed40677394

2025

Deliver CEBPE via cartilage targeting Lipid nanoparticle to block CEBPE-LTF-STAT3 positive feedback loop for efficient treatment of cartilage endplate degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangminghao Zhao,Peichuan Xu,Tao Li,Jinghong Yuan,Wenrui Zhao,Rui Ding,Guoyu Yang,Jiajun Xie,Guanfeng Huang,Zhidong Peng,Xinxin Miao,Xigao Cheng

Discover oncology 16:893 PubMed40410534

2025

Inhibition of colorectal carcinogenesis by sunitinib malate: disruption of the IL-6/STAT3/c-MYC/TWIST/MMP2 autocrine signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Qian,Yi Yang,Bin Zhao,Pan Xu,Ziyan Hu,Liangwang Zhong,Qi Dai,Youbao Zhong,Chao Yang,Qinglong Shu,Ray P S Han,Yang Guan,Zhiming Li,Lai Chen

Open life sciences 20:20221056 PubMed40321161

2025

Convallatoxin inhibits proliferation and angiogenesis of glioma cells via regulating JAK/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongfei Hao,Yaming Han,Yunfei Bo,Liwen Cao,Huijie Fang,Yufei Zhang,Qingbin Li

International journal of women's health 17:1165-1174 PubMed40308269

2025

MiR-484 Regulates the IL-6/STAT3 Signaling Pathway by Down-Regulating KLF12 to Inhibit the Malignant Progression of Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keyong Bao,Xue Zhang,Lihong Bao,Xiaoyu Tao,Li Zhang,Dong Wang

Frontiers in pharmacology 16:1503971 PubMed40206088

2025

Neuroprotective effects of arctigenin on cerebral ischemia-reperfusion injury in rats via the EPO/EPOR-JAK2-STAT5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Xu,Yuting Chen,Lingling Zhang,Wei Lu,Xu Chen,Ting Wang,Wenjie Wang

CytoJournal 22:16 PubMed40134568

2025

Ailanthone inhibits bladder cancer tumor and cell proliferation, epithelial-mesenchymal transition, and activation of the Janus kinase/signal transducer and activator of transcription 3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Li,You Lv,Sheng Xue,Wenyong Li,Xiaole Zhang

Scientific reports 15:9835 PubMed40119074

2025

YTHDF2 promotes the metastasis of oral squamous cell carcinoma through the JAK-STAT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhezheng Chen,Dan Zhao,Yamin Yuan,Lu Zeng,Zhengzhou Luo,Junliang Chen,Xiaorong Lan,Yun He,Lin Liu

Diabetic medicine : a journal of the British Diabetic Association 42:e70014 PubMed40065730

2025

LncRNA CASC2 mediates SOCS6 mRNA stabilization via U2AF2 recruitment to modulate macrophage polarisation in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Xiao,Feipeng Wu,Peipei Zhong,Tian Hu,Rong Luo
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替代产品

Primary Antibodies

AB39636

Anti-JAK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB39636)

  • 5
  • 1
Primary Antibodies

AB195055

Anti-JAK2 (phospho Y1007) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007) antibody (AB195055)

  • 0
  • 0
Primary Antibodies

AB245303

Anti-JAK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (AB245303)

  • 0
  • 0
Primary Antibodies

AB108596

Anti-JAK2 antibody [EPR108(2)]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (AB108596)

  • 4
  • 3

搭配使用产品

Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
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