Anti-JAK2抗体(ab39636)

Thank you for bringing this to our attention. I am sorry to read that two of the antibodies you purchased are not staining blots of your mouse liver samples.

I am assuming the transfer from gel to blot was effective, if your protocol worked with other blots of these samples.

I recommend stripping a blot and re-blocking with 1% BSA without detergent for one hour, and also diluting the antibodies in this 1% BSA solution, instead of non-fat dry milk. I also recommend trying the antibodies at higher concentrations, for instance 1/250 for anti-JAK2 ab39636, and 1/2000 for the phospho-Jak2 antibody, ab32101.

Please let me know the results. If this modification fails, I will be happy to provide replacements, or a credit or refund. The phosphorylation in the liver may be difficult to detect if it is not induced. If possible, I recommend including a positive control such as the treated mouse liver sample shown on the datasheet of ab68268.

Click here (or use the following: https://www.abcam.com/JAK2-phospho-Y1007--Y1008-antibody-ab68268.html).

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

I would like to reassure you that this antibody is tested and covered by our guarantee for IHC-P, IHC-Fr and and in Mouse, Rat, Dog and Human samples.In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

In this case, I woudl be pleased to provide afree of charge replacement or credit note in compensation if the antibody has been used in a tested species.

I would also appreciate if you are able to provide some further information which is beneificial for our quality monitoring records:

1. Which type of tissue has been tested?

2. What dilutions has the antibody been used at?

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for contacting us. The treatment of PFA-fixed, paraffin embedded sections (IHC-P) before incubation with priamry antibody would include antigen retrieval, blocking of endogenous peroxidases (if HRP is being used for detection), and blocking of non-specific proteins. Here are the protocol details for the respective antibodies: ab30646, ab31370, ab39636, ab59389: 1. antigen retrieval: 10 mM citrate buffer (pH6.0) boil in pressure cooker 2. block with 3% H2O2 (in fresh methanol) for 15 minutes at room temperature 3. block with 3% BSA in TBS for 30 minutes ab6672: antigen retrieval: citrate buffer pH 6 and proteinase K digestion have been tried successfully as well as no antigen retrieval. 2. block with 3% hydrogen peroxide for 10 min 3. block with 1-10% Serum for up to 1 hour ab32101: 1. antigen retrieval:10 mM Sodium Citrate Buffer, pH 6.0 in rice cooker 2. block with 3% hydrogen peroxide for 10 min 3. block with PBS + 10% serum I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.