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使用敲除细胞株进行验证重组RabMAb

重组Anti-JAK1抗体[EPR349(N)] (ab133666)

Submit a review Submit a question References (39)
Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
  • Anti-JAK1 antibody [EPR349(N)] (ab133666)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR349(N)] to JAK1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-JAK1抗体[EPR349(N)]
    参阅全部 JAK1 一抗

  • 描述

    兔单克隆抗体[EPR349(N)] to JAK1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Ramos, HT-29, HeLa and Jurkat whole cell lysate (ab7899)

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133666于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 133 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 133 kDa.

靶标

  • 功能

    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

  • 组织特异性

    Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.

  • 结构域

    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.

  • 细胞定位

    Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt

  • 数据库链接

    • 别名

      • JAK 1 antibody
      • JAK 1A antibody
      • JAK 1B antibody
      • JAK-1 antibody
      • JAK1 antibody
      • JAK1_HUMAN antibody
      • JAK1A antibody
      • JAK1B antibody
      • Janus kinase 1 (a protein tyrosine kinase) antibody
      • Janus kinase 1 antibody
      • JTK3 antibody
      • Tyrosine protein kinase JAK 1 antibody
      • Tyrosine protein kinase JAK1 antibody
      • Tyrosine-protein kinase JAK1 antibody
      see all

    图片

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)

      Lane 1: Wild-type HAP1 whole cell lysate (40 µg)              
      Lane 2: Empty
      Lane 3: JAK1 (KO) whole cell lysate (40 µg)
      Lane 4: A431 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab133666 observed at 133 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab133666 was shown to specifically recognize JAK1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when JAK1 knockout samples were examined. Wild-type and JAK1 knockout samples were subjected to SDS-PAGE.  Ab133666 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.         

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 133 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/2000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 133 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified)

      Lane 1 : RAW264.7 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 133 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (purified) + HeLa cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 133 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Western blot - Anti-JAK1 antibody [EPR349(N)] (ab133666)
      All lanes : Anti-JAK1 antibody [EPR349(N)] (ab133666) at 1/1000 dilution (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : HT-29 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 133 kDa

    • Anti-JAK1 antibody [EPR349(N)] (ab133666)
      Anti-JAK1 antibody [EPR349(N)] (ab133666)

    文献 (39)

    发表研究结果有使用 ab133666？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab133666 被引用在 39 文献中.

    • Guo Y  et al. HOTAIR modulates hepatocellular carcinoma progression by activating FUT8/core-fucosylated Hsp90/MUC1/STAT3 feedback loop via JAK1/STAT3 cascade. Dig Liver Dis 55:113-122 (2023). PubMed: 35504805
    • Xu Z  et al. Osteopontin Promotes Macrophage M1 Polarization by Activation of the JAK1/STAT1/HMGB1 Signaling Pathway in Nonalcoholic Fatty Liver Disease. J Clin Transl Hepatol 11:273-283 (2023). PubMed: 36643029
    • Zhang J & Lv G Knockdown of LINC01138 protects human chondrocytes against IL-1β-induced damage by regulating the hsa-miR-1207-5p/KIAA0101 axis. Immun Inflamm Dis 11:e744 (2023). PubMed: 36705420
    • Liu H  et al. Targeting DCLK1 attenuates tumor stemness and evokes antitumor immunity in triple-negative breast cancer by inhibiting IL-6/STAT3 signaling. Breast Cancer Res 25:43 (2023). PubMed: 37069669
    • Ma X  et al. The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 37108290
    View all Publications for this product

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