重组Anti-Jagged1抗体[EPR4290] (ab109536)
![Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)](https://www.abcam.cn/ps/products/109/ab109536/Images/ab109536-490748-anti-jagged1-antibody-epr4290-western-blot-human.jpg "Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4290] to Jagged1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Jagged1抗体[EPR4290]
参阅全部 Jagged1 一抗 -
描述
兔单克隆抗体[EPR4290] to Jagged1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2 and HUVEC whole cell lysate, NIH:OVCAR-3 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4290 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109536于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 134 kDa.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 134 kDa. |
靶标
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功能
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
组织特异性
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
疾病相关
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
序列相似性
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
发展阶段
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 182 Human
- Entrez Gene: 16449 Mouse
- Entrez Gene: 29146 Rat
- Omim: 601920 Human
- SwissProt: P78504 Human
- SwissProt: Q9QXX0 Mouse
- SwissProt: Q63722 Rat
- Unigene: 224012 Human
see all -
别名
- AGS antibody
- AHD antibody
- AWS antibody
see all
图片
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Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: 200, 23 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Jurkat and MOLT-4 whole cell lysate (PMID: 30231940).
200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)](https://www.abcam.cn/ps/products/109/ab109536/Images/ab109536-368948-anti-jagged1-antibody-epr4290-western-blot-knockout-hap1-jag1-human-lysates.jpg)
Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536)All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : JAG1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 134 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 200 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Jagged1 antibody [EPR4290] (ab109536)](https://www.abcam.cn/ps/products/109/ab109536/Images/ab109536-8-benefits-of-recombinant-antibodies.png)
Anti-Jagged1 antibody [EPR4290] (ab109536)
数据表及文件
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SDS download
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Datasheet download
文献 (55)
ab109536 被引用在 55 文献中.
- Zhou YJ et al. Single-cell Transcriptomics Reveals Early Molecular and Immune Alterations Underlying the Serrated Neoplasia Pathway Toward Colorectal Cancer. Cell Mol Gastroenterol Hepatol 15:393-424 (2023). PubMed: 36216310
- Hosoya M et al. Mapping of Notch signaling in the developing organ of Corti in common marmosets. Front Neuroanat 17:1188886 (2023). PubMed: 37351521
- Zhang X et al. Salsolinol improves angiotensin II‑induced myocardial fibrosis in vitro via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway. Exp Ther Med 26:527 (2023). PubMed: 37869646
- Yu L & Li J Punicalagin Alleviates Aged Bronchial Asthma by Inhibiting Th2 Differentiation through IL-4/STAT6 and Jagged1/Notch Pathways. J Healthc Eng 2022:1184677 (2022). PubMed: 35140898
- Hosoya M et al. Early development of the cochlea of the common marmoset, a non-human primate model. Neural Dev 17:6 (2022). PubMed: 35524278