重组Anti-Jagged1抗体[EPR23647-75] - BSA and Azide free (ab273578)
![Flow Cytometry - Anti-Jagged1 antibody [EPR23647-75] - BSA and Azide free (ab273578)](https://www.abcam.cn/ps/products/273/ab273578/Images/ab273578-1-anti-jagged1-antibody-epr2364775-flow-cytometry-jurkat.jpg "Flow Cytometry - Anti-Jagged1 antibody [EPR23647-75] - BSA and Azide free (ab273578)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23647-75] to Jagged1 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Jagged1抗体[EPR23647-75] - BSA and Azide free
参阅全部 Jagged1 一抗 -
描述
兔单克隆抗体[EPR23647-75] to Jagged1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cytmore details
不适用于: ICC/IF,IHC-P,IP or WB -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: HepG2 cells.
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常规说明
ab273578 is the carrier-free version of ab273571.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23647-75 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
组织特异性
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
疾病相关
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
序列相似性
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
发展阶段
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 182 Human
- Omim: 601920 Human
- SwissProt: P78504 Human
- Unigene: 224012 Human
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别名
- AGS antibody
- AHD antibody
- AWS antibody
see all
图片
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Flow Cytometry - Anti-Jagged1 antibody [EPR23647-75] - BSA and Azide free (ab273578)
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Jagged1 with ab273571 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat (PMID: 30231940).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273571).
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![Anti-Jagged1 antibody [EPR23647-75] - BSA and Azide free (ab273578)](https://www.abcam.cn/ps/products/273/ab273578/Images/ab273578-2-benefits-of-recombinant-antibodies.png)
Anti-Jagged1 antibody [EPR23647-75] - BSA and Azide free (ab273578)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab273578 尚未被引用在任何文献中。
