重组Anti-JAB1抗体[EPR1350] (ab124720)
![OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (ab124720)](https://www.abcam.cn/ps/products/124/ab124720/Images/ab124720-138532-anti-jab1-antibody-epr1350.jpg "OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (ab124720)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1350] to JAB1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-JAB1抗体[EPR1350]
参阅全部 JAB1 一抗 -
描述
兔单克隆抗体[EPR1350] to JAB1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human JAB1 aa 1-100. The exact sequence is proprietary.
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阳性对照
- NIH 3T3, U87-MG, MCF7, and PC12 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 6.45 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1350 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124720于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
应用说明
Is unsuitable for Flow Cyt.
靶标
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功能
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. -
序列相似性
Belongs to the peptidase M67A family. CSN5 subfamily.
Contains 1 MPN (JAB/Mov34) domain. -
结构域
The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10987 Human
- Entrez Gene: 26754 Mouse
- Entrez Gene: 312916 Rat
- Omim: 604850 Human
- SwissProt: Q92905 Human
- SwissProt: O35864 Mouse
- Unigene: 491912 Human
- Unigene: 402384 Mouse
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别名
- 38 kDa Mov34 homolog antibody
- COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
- COP9 constitutive photomorphogenic homolog subunit 5 antibody
see all
图片
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OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (ab124720)
-
![Western blot - Anti-JAB1 antibody [EPR1350] (ab124720)](https://www.abcam.cn/ps/products/124/ab124720/Images/ab124720-138533-anti-jab1-antibody-epr1350-western-blot.jpg)
Western blot - Anti-JAB1 antibody [EPR1350] (ab124720)All lanes : Anti-JAB1 antibody [EPR1350] (ab124720) at 1/1000 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
![Anti-JAB1 antibody [EPR1350] (ab124720)](https://www.abcam.cn/ps/products/124/ab124720/Images/ab124720-3-benefits-of-recombinant-antibodies.png)
Anti-JAB1 antibody [EPR1350] (ab124720)
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab124720 被引用在 10 文献中.
- Yao W et al. Activation of Esterase D by FPD5 Inhibits Growth of A549 Lung Cancer Cells via JAB1/p53 Pathway. Genes (Basel) 13:N/A (2022). PubMed: 35627173
- Yuan L et al. 18β-glycyrrhetinic acid regulates mitochondrial ribosomal protein L35-associated apoptosis signaling pathways to inhibit proliferation of gastric carcinoma cells. World J Gastroenterol 28:2437-2456 (2022). PubMed: 35979263
- Ruan Z et al. Shikonin-mediated PD-L1 degradation suppresses immune evasion in pancreatic cancer by inhibiting NF-?B/STAT3 and NF-?B/CSN5 signaling pathways. Pancreatology 21:630-641 (2021). PubMed: 33707115
- Wang M et al. Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation. Biomaterials 225:119539 (2019). PubMed: 31622821
- Hernández-Puga G et al. Jab1 is a T2-dependent coactivator or a T3-dependent corepressor of TRB1-mediated gene regulation. J Endocrinol 232:451-459 (2017). PubMed: 28053002
- Coleman KE et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). PubMed: 28475037
- Wang R et al. COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain. J Biol Chem 290:9299-309 (2015). Mouse . PubMed: 25713139
- Nie W et al. miR-1470 mediates lapatinib induced p27 upregulation by targeting c-jun. J Cell Physiol 230:1630-9 (2015). PubMed: 25545366
- Wang H et al. COPS5 (Jab1) protein increases ß site processing of amyloid precursor protein and amyloid ß peptide generation by stabilizing RanBP9 protein levels. J Biol Chem 288:26668-77 (2013). PubMed: 23926111
- He F et al. X-linked intellectual disability gene CUL4B targets Jab1/CSN5 for degradation and regulates bone morphogenetic protein signaling. Biochim Biophys Acta 1832:595-605 (2013). PubMed: 23357576