AB32039

重组Anti-ITK/EMT抗体[Y401]

Name: ITK/EMT抗体[Y401] (ab32039)| Abcam中文官网
Brand: Abcam Limited
SKU: AB32039
Price: 2783 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-ITK/EMT antibody [Y401]

RabMAb
Recombinant
了解详情

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal ITK/EMT antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.

查看别名

EMT, LYK, ITK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, IL-2-inducible T-cell kinase

3 Images

Flow Cytometry (Intracellular) - Anti-ITK/EMT antibody [Y401] (AB32039)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ITK/EMT antibody [Y401] (AB32039)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ITK/EMT with purified ab320396 at 1 : 50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

Unknown

Western blot - Anti-ITK/EMT antibody [Y401] (AB32039)

All lanes:

Western blot - Anti-ITK/EMT antibody [Y401] (ab32039) at 1/5000 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 72 kDa

Observed band size: 68 kDa

false

Lab

Western blot - Anti-ITK/EMT antibody [Y401] (AB32039)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control.

All lanes:

Western blot - Anti-ITK/EMT antibody [Y401] (ab32039) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 68 kDa

false

不同偶联物与剂型 (9)

Carrier free

Anti-ITK/EMT antibody [Y401] - BSA and Azide free
660 APC

APC Anti-ITK / EMT antibody [Y401]
HRP

HRP Anti-ITK / EMT antibody [Y401]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ITK / EMT antibody [Y401]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ITK / EMT antibody [Y401]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-ITK / EMT antibody [Y401]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ITK / EMT antibody [Y401]
578 PE

PE Anti-ITK / EMT antibody [Y401]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ITK / EMT antibody [Y401]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Y401

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ITK also known as Interleukin-2-inducible T-cell kinase is a protein belonging to the Tec family of non-receptor tyrosine kinases. It weighs approximately 72 kDa and plays a significant role in signal transduction in immune cells specifically T cells. ITK is widely expressed in lymphoid tissues particularly in thymocytes and peripheral T cells. ITK products often include variants like ITK-1 and ITK kit which are useful in various scientific applications.

Biological function summary

ITK is integral to the activation and development of T cells which are critical components of the adaptive immune response. ITK functions as part of a larger signaling complex interacting with proteins such as LAT and SLP-76 to mediate downstream signaling events following T-cell receptor engagement. This interaction facilitates the phosphorylation of multiple substrates which is necessary for T-cell proliferation differentiation and cytokine production.

Pathways

ITK plays an important role in T-cell receptor signaling and the downstream Ras/MAPK pathway. It interacts with proteins like ZAP-70 and LCK in these pathways which are important for initiating T-cell activation and function. The modulation of these pathways by ITK is essential for proper immune function and response to antigens.

ITK is linked to immunodeficiency and autoimmune disorders. Dysregulation of ITK activity can lead to conditions like X-linked lymphoproliferative syndrome and systemic lupus erythematosus. The EMT protein also ties with ITK concerning certain neoplastic conditions where aberrant signaling contributes to the pathogenesis. Researchers study ITK's interactions with other proteins such as BSA EMT to understand its role in these diseases better and explore potential therapeutic targets.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins : the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed : 12186560, PubMed : 12682224, PubMed : 21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).

See full target information ITK

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 220: PubMed36326697

2022

Inherited human ITK deficiency impairs IFN-γ immunity and underlies tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Masato Ogishi,Rui Yang,Rémy Rodriguez,Dominic P Golec,Emmanuel Martin,Quentin Philippot,Jonathan Bohlen,Simon J Pelham,Andrés Augusto Arias,Taushif Khan,Manar Ata,Fatima Al Ali,Flore Rozenberg,Xiao-Fei Kong,Maya Chrabieh,Candice Laine,Wei-Te Lei,Ji Eun Han,Yoann Seeleuthner,Zenia Kaul,Emmanuelle Jouanguy,Vivien Béziat,Leila Youssefian,Hassan Vahidnezhad,V Koneti Rao,Bénédicte Neven,Claire Fieschi,Davood Mansouri,Mohammad Shahrooei,Sevgi Pekcan,Gulsum Alkan,Melike Emiroğlu,Hüseyin Tokgöz,Jouni Uitto,Fabian Hauck,Jacinta Bustamante,Laurent Abel,Sevgi Keles,Nima Parvaneh,Nico Marr,Pamela L Schwartzberg,Sylvain Latour,Jean-Laurent Casanova,Stéphanie Boisson-Dupuis

Aging 13:18620-18644 PubMed34282055

2021

Interleukin-2 inducible T-cell kinase: a potential prognostic biomarker and tumor microenvironment remodeling indicator for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Binhua Pan,Modan Yang,Xuyong Wei,Wangyao Li,Kun Wang,Mengfan Yang,Di Lu,Rui Wang,Beini Cen,Xiao Xu

Cancers 13: PubMed34283052

2021

Possible Therapeutic Strategy Involving the Purine Synthesis Pathway Regulated by ITK in Tongue Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kaoru Onidani,Nami Miura,Yuki Sugiura,Yuichi Abe,Yukio Watabe,Takanori Kakuya,Taisuke Mori,Seiichi Yoshimoto,Jun Adachi,Takao Kiyoi,Yasuaki Kabe,Makoto Suematsu,Takeshi Tomonaga,Takahiko Shibahara,Kazufumi Honda

Experimental and therapeutic medicine 19:2887-2894 PubMed32256773

2020

Blockade of the mTOR signaling pathway with rapamycin ameliorates aristolochic acid nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Wu,Lili Tang,Bicheng Chen,Jianjian Zheng,Fengquan Dong,Zhen Su,Fan Lin

Cancer cell international 19:32 PubMed30814910

2019

ITK inhibition induced in vitro and in vivo anti-tumor activity through downregulating TCR signaling pathway in malignant T cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yalu Liu,Xiaogan Wang,Lijuan Deng,Lingyan Ping,Yunfei Shi,Wen Zheng,Ningjing Lin,Xiaopei Wang,Meifeng Tu,Yan Xie,Weiping Liu,Zhitao Ying,Chen Zhang,Zhengying Pan,Xi Wang,Ning Ding,Yuqin Song,Jun Zhu

Histopathology 73:1030-1038 PubMed30102799

2018

Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Miyata-Takata,Shih-Sung Chuang,Katsuyoshi Takata,Tomohiro Toji,Yoshinobu Maeda,Yasuharu Sato,Tadashi Yoshino

Scientific reports 8:14216 PubMed30242208

2018

Comparison of interleukin-2-inducible kinase (ITK) inhibitors and potential for combination therapies for T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sami Mamand,Rebecca L Allchin,Matthew J Ahearne,Simon D Wagner

Clinical cancer research : an official journal of the American Association for Cancer Research 21:2167-76 PubMed25934889

2015

IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma.

Applications

Unspecified application

Species

Human

Craig C Carson,Stergios J Moschos,Sharon N Edmiston,David B Darr,Nana Nikolaishvili-Feinberg,Pamela A Groben,Xin Zhou,Pei Fen Kuan,Shaily Pandey,Keefe T Chan,Jamie L Jordan,Honglin Hao,Jill S Frank,Dennis A Hopkinson,David C Gibbs,Virginia D Alldredge,Eloise Parrish,Sara C Hanna,Paula Berkowitz,David S Rubenstein,C Ryan Miller,James E Bear,David W Ollila,Norman E Sharpless,Kathleen Conway,Nancy E Thomas

Virology 436:235-43 PubMed23260110

2012

Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization.

Applications

Unspecified application

Species

Unspecified reactive species

Gillian M Schiralli Lester,Hisashi Akiyama,Erica Evans,Juswinder Singh,Suryaram Gummuluru,Andrew J Henderson

Haematologica 96:472-6 PubMed21109689

2010

IL-2-inducible T-cell kinase deficiency: clinical presentation and therapeutic approach.

Applications

Species

Human

Polina Stepensky,Michael Weintraub,Asaf Yanir,Shoshana Revel-Vilk,Frank Krux,Kirsten Huck,Rene M Linka,Avraham Shaag,Orly Elpeleg,Arndt Borkhardt,Igor B Resnick

View all publications

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重组Anti-ITK/EMT抗体[Y401]