AB316250

重组Anti-ITGA11抗体[EPR28689-21] - BSA and Azide free

Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
KO Validated
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Knockout Tested Rabbit Recombinant Monoclonal ITGA11 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

查看别名

MSTP018, ITGA11, Integrin alpha-11

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ITGA11 with ab316249 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in tumor stroma interphase on Human breast carcinoma (PMID : 31605508).
The section was incubated with ab316249 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling ITGA11 with ab316249 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in tumor stroma interphase on human cervical squamous carcinoma (PMID : 36003785).
The section was incubated with ab316249 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ITGA11 with ab316249 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human spleen.
The section was incubated with ab316249 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Western blot : Anti-ITGA11 antibody (ab316249) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5]

(ab8245) loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab316249 was shown to bind specifically to ITGA11. Target of interest was observed at 160 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in ITGA11 knockout cell line (lane 2, knockout cell line ab265494 / knockout cell lysate ab258929). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

Negative control : A431 (PMID : 31605508).

All lanes:

Western blot - Anti-ITGA11 antibody [EPR28689-21] (<a href='/products/primary-antibodies/itga11-antibody-epr28689-21-ab316249'>ab316249</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

ITGA11 knockout HeLa whole cell lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

Observed band size: 160 kDa,36 kDa

false

Supplier Data

Western blot - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Negative control : 293T (PMID : 31605508).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ITGA11 antibody [EPR28689-21] (<a href='/products/primary-antibodies/itga11-antibody-epr28689-21-ab316249'>ab316249</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 160 kDa,36 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-ITGA11 antibody [EPR28689-21] - BSA and Azide free (AB316250)

This data was developed using ab316249, the same antibody clone in a different buffer formulation.

Negative control : 4T1 (PMID : 26990302), C2C12 (PMID : 11518510).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ITGA11 antibody [EPR28689-21] (<a href='/products/primary-antibodies/itga11-antibody-epr28689-21-ab316249'>ab316249</a>) at 1/1000 dilution

Lane 1:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 160 kDa,36 kDa

false

Exposure time: 37s

不同偶联物与剂型 (1)

Unconjugated

Anti-ITGA11 antibody [EPR28689-21]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28689-21

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Not suitable for mouse IHC-P

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab316250 is the carrirer-free version of ab316249.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ITGA11 also known as Integrin alpha-11 functions mechanically as an alpha subunit that combines with the beta-1 subunit to form a collagen receptor integrin complex. The protein has a mass of approximately 135 kDa and its expression occurs mainly in fibroblastic cells located within connective tissue including areas like skin and lungs. ITGA11 plays a role in cell adhesion helping mediate interactions between cells and the extracellular matrix which is essential for tissue structure and function.

Biological function summary

ITGA11 influences various cellular processes including cell migration proliferation and differentiation. The protein is part of the alpha-11 beta-1 integrin complex that specifically binds to interstitial collagens. This interaction allows ITGA11 to facilitate communication signals that control cellular behavior. Its effective function in collagen recognition establishes ITGA11 as important for maintaining the structural integrity and repair of tissues particularly during injury or developmental processes.

Pathways

ITGA11 integrates into the collagen-mediated signaling pathway which connects to cellular responses to the extracellular matrix. In particular ITGA11 interacts with other proteins like integrin beta-1 to affect cell survival and movement. Another important pathway involving ITGA11 is the TGF-beta signaling pathway where it contributes to matrix reorganization and fibroblast activation both significant in tissue development and repair mechanisms.

ITGA11 shows association with fibrotic diseases and certain cancers. In fibrosis excessive collagen deposition occurs and ITGA11's interaction with collagen and its regulatory role in fibroblasts can exacerbate this condition. This relationship links ITGA11 to proteins like integrin beta-1 and TGF-beta which are involved in fibrotic tissue transformation. In cancer ITGA11 supports tumor stroma formation contributing to cancer cell survival and spread emphasizing its importance in tumor biology and as a potential therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integrin alpha-11/beta-1 is a receptor for collagen.

See full target information ITGA11

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-ITGA11抗体[EPR28689-21] - BSA and Azide free