Anti-ISY1抗体(ab121250)
Key features and details
- Rabbit polyclonal to ISY1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ISY1抗体 -
描述
兔多克隆抗体to ISY1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to amino acids 23-146 of Human ISY1 (Q9ULR0).
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阳性对照
- WB: U-251 MG, RT4, NIH-3T3, and NBT-II whole cell lysates; IHC-P: Human lymphoid, testis, placenta, and kidney tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121250于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
靶标
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功能
May play a role in pre-mRNA splicing. -
序列相似性
Belongs to the ISY1 family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 57461 Human
- Entrez Gene: 57905 Mouse
- Entrez Gene: 362394 Rat
- Omim: 612764 Human
- SwissProt: Q9ULR0 Human
- SwissProt: Q69ZQ2 Mouse
- SwissProt: Q6AYB3 Rat
- Unigene: 512661 Human
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别名
- FSAP33 antibody
- ISY1 antibody
- ISY1 splicing factor homolog (S. cerevisiae) antibody
see all
图片
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Western blot - Anti-ISY1 antibody (ab121250)All lanes : Anti-ISY1 antibody (ab121250)
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISY1 antibody (ab121250)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of kidney tissue labelling ISY1 with ab121250 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-ISY1 antibody (ab121250)All lanes : Anti-ISY1 antibody (ab121250)
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat urinary bladder tumor cell line) whole cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISY1 antibody (ab121250)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling ISY1 with ab121250 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISY1 antibody (ab121250)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ISY1 with ab121250 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISY1 antibody (ab121250)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphoid tissue labelling ISY1 with ab121250 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121250 被引用在 2 文献中.
- Du P et al. An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition. Cell Stem Cell 22:851-864.e5 (2018). PubMed: 29804889
- Guilgur LG et al. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3:e02181 (2014). WB . PubMed: 24755291