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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin

Anti-Isopeptide抗体[81D1C2] (ab422)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (10)

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Key features and details

  • Mouse monoclonal [81D1C2] to Isopeptide
  • Suitable for: WB
  • Reacts with: Species independent
  • Isotype: IgG1

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Anti-N epsilon gamma glutamyl Lysine antibody [81D4] (ab424)

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概述

  • 产品名称

    Anti-Isopeptide抗体[81D1C2]
  • 描述

    小鼠单克隆抗体[81D1C2] to Isopeptide
  • 宿主

    Mouse
  • 特异性

    This antibody reacts with the isopeptide : epsilon-(gamma-glutamyl) lysine. There is no cross reaction with either lysine or glutamine. They are specific to the epsilon-(gamma-glutamyl) lysine bond formed by transglutaminase activity.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic isopeptide.

  • 常规说明

    The ability of this antibody to fix complement is unknown. The isopeptide may be purchased from Bachem Catalog number G-1970.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    81D1C2
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab422于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/50 - 1/500.
说明
WB
1/50 - 1/500.

靶标

  • 别名

    • Isopeptide bond antibody

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (10)

发表研究结果有使用 ab422?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab422 被引用在 10 文献中.

  • Schopfer LM & Lockridge O Chlorpyrifos oxon promotes tubulin aggregation via isopeptide cross-linking between diethoxyphospho-Lys and Glu or Asp: Implications for neurotoxicity. J Biol Chem 293:13566-13577 (2018). PubMed: 30006344
  • Liu C  et al. Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification. Hypertension 63:353-61 (2014). WB . PubMed: 24191290
  • Llano E  et al. STAG3 is a strong candidate gene for male infertility. Hum Mol Genet 23:3421-31 (2014). PubMed: 24608227
  • Nicoll WS  et al. Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase. Malar J 10:14 (2011). PubMed: 21255444
  • L'Heureux DZ  et al. The interaction of angiocidin with tissue transglutaminase. Exp Mol Pathol 88:15-25 (2010). PubMed: 19931242
  • Chlenski A  et al. SPARC expression is associated with impaired tumor growth, inhibited angiogenesis and changes in the extracellular matrix. Int J Cancer 118:310-6 (2006). PubMed: 16052522
  • Mangala LS  et al. Tissue transglutaminase-induced alterations in extracellular matrix inhibit tumor invasion. Mol Cancer 4:33 (2005). PubMed: 16153302
  • Johnson GV & LeShoure R Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity. J Neurosci Methods 134:151-8 (2004). PubMed: 15003381
  • el Alaoui S  et al. Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study. Int J Cancer 48:221-6 (1991). PubMed: 1673452
  • Roch AM  et al. Differential expression of isopeptide bonds N epsilon (gamma-glutamyl) lysine in benign and malignant human breast lesions: an immunohistochemical study. Int J Cancer 48:215-20 (1991). PubMed: 1673451

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Isopeptide antibody [81D1C2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human umbilical artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH=8
Permeabilization
No
Specification
Human umbilical artery
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Prof. Jin-Jia Hu

Verified customer

提交于 May 11 2015

Question

Customer has purchased ab422 and needs to know the following information about this antibody: how was the antigen generated? Was cross-linking (such as BSA) involved in generating the peptide? To generate a control, what should the antibody be pre-absorbed with? Any information to help her would be greatly appreciated! Thank you in advance!

Read More

Abcam community

Verified customer

Asked on Dec 15 2003

Answer

Antigen: BSA; ab422 is a monoclonal and does not need to be pre-absorbed.

Read More

Abcam Scientific Support

回复于 Dec 31 2003

Question

Hi I`m using your ab 422. Two questions. Do you know if this antibody could be used in flow cytometry analysis? and is there a directly labeled (ex. FITC) version of this antibody? I`m also working with AB8828, is this Ab. suitable for flow studies? Thanks

Read More

Abcam community

Verified customer

Asked on Mar 25 2002

Answer

As far as we are aware, ab422 has not yet been tested in in flow cytometry. We do not stock a labelled version of this antibody. ab8828 also has not yet been tested in flow cytometry. If you do test these antibodies in FC, please let us know how you get on by submitting a summary of your results via the reviews section on the datasheets. In return we will forward a reward of your choice -- typically an Amazon gift voucher.

Read More

Abcam Scientific Support

回复于 Mar 25 2002

Question

I am using Ab422, the antibody that recognizes N-epsilon(gamma-glutamyl)Lys. To do appropriate control I need to get N-epsilon(gamma-glutamyl)Lys itself. Do you sell this peptide? Where to get it from?

Read More

Abcam community

Verified customer

Asked on Jun 26 2001

Answer

The MAb to isopeptide shows good reactivity on cross linked protein containing isopeptide N-epsilon(gamma-glutamyl)Lys but has weak reactivity on free isopeptide. Therefore commercialy available free isopeptide from Sigma Chemical is not a good positive control.

Read More

Neil Hayward

Abcam Scientific Support

回复于 Jun 26 2001

Question

Please explain any differences between 153-81D4 and -81D1C2. I wish to perform both western blotting and immunohistochemistry for the isopeptide.

Read More

Abcam community

Verified customer

Asked on Mar 26 2001

Answer

The difference between these two antibodies is the isotype: 153-81D4 is an IgM and 153-81D1C2 is an IgG1. They can both be used for both applications, so the choice is yours.

Read More

Abcam Scientific Support

回复于 Mar 27 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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