重组Anti-Islet 1抗体[EP4182] - Neural Stem Cell Marker (ab109517)
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251148-anti-islet-1-antibody-ep4182-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Chicken, Human
Related conjugates and formulations
概述
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产品名称
Anti-Islet 1抗体[EP4182] - Neural Stem Cell Marker
参阅全部 Islet 1 一抗 -
描述
兔单克隆抗体[EP4182] to Islet 1 - Neural Stem Cell Marker -
宿主
Rabbit -
特异性
86% identities with Islet 2 -
经测试应用
适用于: ICC/IF, WB, IP, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Chicken, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
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常规说明
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.
Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP4182 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203404)
- Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203405)
- Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
- Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
- Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
- Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab277925)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109517于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/250.
For unpurified use at 1/50 - 1/100. |
| WB |
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
|
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| IP |
1/10 - 1/100.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
1/250. For unpurified use at 1/50 - 1/100. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa. |
|
IP
1/10 - 1/100. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Binds to one of the cis-acting domain of the insulin gene enhancer. -
组织特异性
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
序列相似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- Unigene: 505 Human
- Unigene: 42242 Mouse
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别名
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-122431-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251524-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251523-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-210993-anti-islet-1-antibody-ep4182-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
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![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-268754-anti-islet-1-antibody-ep4182-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251522-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251521-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-251246-anti-islet-1-antibody-ep4182-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-210490-anti-islet-1-antibody-ep4182-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
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![Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.cn/ps/products/109/ab109517/Images/ab109517-13-benefits-of-recombinant-antibodies.png)
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab109517 被引用在 59 文献中.
- Pintacuda G et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
- Hsu YH et al. Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia. iScience 26:106701 (2023). PubMed: 37207277
- Ho WY et al. FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons. iScience 26:108152 (2023). PubMed: 37920668
- Zheng X et al. Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells. iScience 26:108306 (2023). PubMed: 38026209
- Tay SH et al. Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells. Methods Mol Biol 2549:359-377 (2022). PubMed: 33959917