重组Anti-ISL-2抗体[EPR12785] (ab176576)
![Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)](https://www.abcam.cn/ps/products/176/ab176576/Images/ab176576-204273-anti-isl-2-antibody-epr12785-western-blot.jpg "Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12785] to ISL-2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ISL-2抗体[EPR12785]
参阅全部 ISL-2 一抗 -
描述
兔单克隆抗体[EPR12785] to ISL-2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Zebrafish
不与反应:
Mouse, Rat -
免疫原
Synthetic peptide within Human ISL-2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96A47 -
阳性对照
- 293T, HeLa and HUVEC cell line lysates; 293 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12785 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
靶标
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功能
Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways. -
序列相似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 64843 Human
- Entrez Gene: 30157 Zebrafish
- Omim: 609481 Human
- SwissProt: Q96A47 Human
- SwissProt: P53406 Zebrafish
- Unigene: 444677 Human
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别名
- FLJ10160 antibody
- Insulin gene enhancer protein ISL 2 antibody
- Insulin gene enhancer protein ISL-2 antibody
see all
图片
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Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)All lanes : Anti-ISL-2 antibody [EPR12785] (ab176576) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa -
![Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (ab176576)](https://www.abcam.cn/ps/products/176/ab176576/Images/ab176576-204272-anti-isl-2-antibody-epr12785-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (ab176576)Intracellular flow cytometric analysis of permeabilized 293 cells labeling Islet 2 with ab176576 at a 1/10 dilution (red), or a rabbit IgG negative control (green).
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![Anti-ISL-2 antibody [EPR12785] (ab176576)](https://www.abcam.cn/ps/products/176/ab176576/Images/ab176576-3-benefits-of-recombinant-antibodies.png)
Anti-ISL-2 antibody [EPR12785] (ab176576)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab176576 尚未被引用在任何文献中。