AB249340

重组Anti-ISG20抗体[EPR8973] - BSA and Azide free

Anti-ISG20 antibody [EPR8973] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ISG20 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

查看别名

HEM45, ISG20, Interferon-stimulated gene 20 kDa protein, Estrogen-regulated transcript 45 protein, Promyelocytic leukemia nuclear body-associated protein ISG20

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody [EPR8973] - BSA and Azide free (AB249340)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody [EPR8973] - BSA and Azide free (AB249340)

This data was developed using ab157477, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ISG20 with ab157477 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody [EPR8973] - BSA and Azide free (AB249340)

This data was developed using ab157477, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling ISG20 with ab157477 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ISG20 antibody [EPR8973] - BSA and Azide free (AB249340)

This data was developed using ab157477, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ISG20 antibody [EPR8973] (<a href='/products/primary-antibodies/isg20-antibody-epr8973-ab157477'>ab157477</a>) at 1/1000 dilution

All lanes:

Daudi cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-ISG20 antibody [EPR8973]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR8973

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab249340 is the carrier-free version of ab157477.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ISG20 also known as Interferon Stimulated Gene 20 kDa is an exonuclease enzyme with a molecular weight of around 20 kilodaltons. It degrades single-stranded RNA and DNA primarily involved in antiviral defense by targeting viral RNAs. ISG20 is widely expressed in several human tissues with high expression levels in immune-related cells and tissues such as lymph nodes spleen and blood leukocytes. This protein becomes strongly upregulated in response to interferons linking it to the body's innate immune response.

Biological function summary

ISG20 serves as an antiviral factor by degrading viral genomes to restrict infection. It does not work alone; often it functions as part of a larger antiviral mechanism alongside other interferon-induced proteins like PKR and OAS. Through its RNA-degrading activity ISG20 contributes significantly to controlling the spread of viruses by breaking down the RNA from invading organisms particularly RNA viruses. This makes ISG20 an important player in reducing viral loads during infections.

Pathways

ISG20 is mainly associated with the interferon signaling pathway where it acts downstream to execute immune processes. It interacts with and complements other antiviral proteins such as RNase L which also plays a role in degrading RNA during viral responses. Additionally ISG20 links to the broader regulation of apoptosis and cell death pathways showing how it assists in eliminating infected cells to prevent further viral proliferation.

ISG20 has implications in viral infections such as hepatitis C and influenza. Its role in degrading viral RNA positions it as a potential therapeutic target for mitigating these infections. During hepatitis C infection ISG20 works in conjunction with proteins like IRF9 to modulate immune response and limit virus replication. Researchers study ISG20 for its potential contribution to the severity of flu infections where its activity may influence the immune system's effectiveness in combating the influenza virus.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Interferon-induced antiviral exoribonuclease that acts mainly on single-stranded RNA (PubMed : 11401564, PubMed : 12594219, PubMed : 16033969). Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) (PubMed : 16514659, PubMed : 21036379). Inhibition of several viruses such as chikungunya virus (CHIKV) does not involve the degradation of viral RNAs, but rather the inhibition of translation of viral proteins (By similarity). Exerts a translational control over a large panel of non-self RNA substrates while sparing endogenous transcripts. This activity correlates with the protein's ability to localize in cytoplasmic processing bodies (PubMed : 31600344). May also act as master regulator of over hundred interferon stimulated genes leading to viral genome translation inhibition (By similarity). May play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (PubMed : 16514659).

See full target information ISG20

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重组Anti-ISG20抗体[EPR8973] - BSA and Azide free