Anti-IRX2抗体(ab72975)
Key features and details
- Mouse polyclonal to IRX2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IRX2抗体
参阅全部 IRX2 一抗 -
描述
小鼠多克隆抗体to IRX2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human IRX2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Hela cells and IRX2 transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72975于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ICC/IF |
| 说明 |
|---|
Protein A purify ab72975 before starting ICC/IF procedure.
WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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序列相似性
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 153572 Human
- Omim: 606198 Human
- SwissProt: Q9BZI1 Human
- Unigene: 282089 Human
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别名
- Homeodomain protein IRXA2 antibody
- Iroquois homeobox 2 antibody
- Iroquois homeobox protein 2 antibody
see all
图片
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Western blot - Anti-IRX2 antibody (ab72975)All lanes : Anti-IRX2 antibody (ab72975) at 1/500 dilution
Lane 1 : IRX2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-IRX2 antibody (ab72975)HeLa cells with Protein A purified ab72975 at 10ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab72975 被引用在 2 文献中.
- Liu T et al. Lineage conversion of mouse fibroblasts to pancreatic a-cells. Exp Mol Med 49:e350 (2017). ICC/IF . PubMed: 28665920
- Liu T et al. IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner. Mol Med Rep 12:4346-4351 (2015). PubMed: 26062523