Anti-IRSp53抗体- C-terminal (ab15697)
Key features and details
- Goat polyclonal to IRSp53 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
概述
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产品名称
Anti-IRSp53抗体- C-terminal
参阅全部 IRSp53 一抗 -
描述
山羊多克隆抗体to IRSp53 - C-terminal -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Dog, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human IRSp53 aa 512-520 (C terminal).
Sequence:C-SGSGTLVSTV
(Peptide available asab23256) -
阳性对照
- WB: Human brain lysate.
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常规说明
This antibody is expected to recognise the human isoform 1 only of this protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
This antibody is expected to recognise the human isoform 1 only of this protein. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. -
组织特异性
Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta, prostate and testis. A short isoform is ubiquitous, with the highest expression in liver, prostate, testis and placenta. -
序列相似性
Contains 1 IMD (IRSp53/MIM homology) domain.
Contains 1 SH3 domain. -
结构域
The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
The SH3 domain interacts with ATN1, BAI1, WASF1, WASF2, SHANK1, DIAPH1 and ENAH. -
翻译后修饰
Phosphorylated on tyrosine residues by INSR in response to insulin treatment. -
细胞定位
Cytoplasm. Membrane. Cell projection > filopodium. Cell projection > ruffle. Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 403473 Dog
- Entrez Gene: 10458 Human
- Entrez Gene: 108100 Mouse
- Entrez Gene: 117542 Rat
- GenBank: NP_006331.1 Human
- Omim: 605475 Human
- SwissProt: Q9UQB8 Human
- SwissProt: Q8BKX1 Mouse
see all -
别名
- BAI-associated protein 2 antibody
- BAI1 associated protein 2 antibody
- BAI1-associated protein 2 antibody
see all
图片
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Western blot - Anti-IRSp53 antibody - C-terminal (ab15697)Anti-IRSp53 antibody - C-terminal (ab15697) at 1 µg/ml + Lysates prepared from human brain at 35 µg
Predicted band size: 60 kDaab15697 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
数据表及文件
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Datasheet download
文献 (3)
ab15697 被引用在 3 文献中.
- Fedoryshchak RO et al. Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme. Elife 9:N/A (2020). PubMed: 32975518
- Goodwin JM et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). PubMed: 25042806
- Cohen D et al. The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling. J Cell Biol 192:525-40 (2011). WB ; Dog . PubMed: 21282462
