重组Anti-IRS4抗体[EP907Y] (ab52622)
![Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)](https://www.abcam.cn/ps/products/52/ab52622/Images/ab52622-450781-anti-irs4-antibody-ep907y-westernblot-hek-293-human.jpg "Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP907Y] to IRS4
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRS4抗体[EP907Y]
参阅全部 IRS4 一抗 -
描述
兔单克隆抗体[EP907Y] to IRS4 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK-293 whole cell lysate; IP: HEK-293 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP907Y -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52622于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
For unpurified use at 1/5000 dilution |
|
| IP |
1/20.
|
| 说明 |
|---|
|
WB
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa). For unpurified use at 1/5000 dilution |
|
IP
1/20. |
靶标
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相关性
IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. -
细胞定位
Cell membrane; Peripheral membrane protein; Cytoplasmic side. -
数据库链接
- Entrez Gene: 8471 Human
- Omim: 603510 Human
- SwissProt: O14654 Human
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别名
- 160 kDa phosphotyrosine protein antibody
- Insulin receptor substrate 4 antibody
- IRS 4 antibody
- PY160 antibody
图片
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Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)Anti-IRS4 antibody [EP907Y] (ab52622) at 1/10000 dilution (Purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDaBlocking buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (ab52622)](https://www.abcam.cn/ps/products/52/ab52622/Images/ab52622-4-anti-irs4-antibody-ep907y-immunoprecipitation-hek293-human.jpg)
Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (ab52622)Purified ab52622 at 1/20 dilution (0.5µg) immunoprecipitating IRS4 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52622 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52622 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 133 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab52622 被引用在 8 文献中.
- Yang F et al. Long Noncoding RNA LINC00173 Promotes the Malignancy of Melanoma by Promoting the Expression of IRS4 Through Competitive Binding to microRNA-493. Cancer Manag Res 12:3131-3144 (2020). PubMed: 32440211
- Li X et al. Phosphorylation of IRS4 by CK1?2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway. Theranostics 8:3643-3653 (2018). PubMed: 30026872
- Weischenfeldt J et al. Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking. Nat Genet 49:65-74 (2017). PubMed: 27869826
- Cui A et al. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression. Tumour Biol 39:1010428317701640 (2017). PubMed: 28475006
- Homma Y et al. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem 289:26302-13 (2014). PubMed: 25100728
- Chai B et al. A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signaling. J Neuroendocrinol : (2012). PubMed: 22935015
- Mertens F et al. The t(X;6) in subungual exostosis results in transcriptional deregulation of the gene for insulin receptor substrate 4. Int J Cancer 128:487-91 (2011). PubMed: 20340132
- Karrman K et al. The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in overexpression of the insulin receptor substrate 4 gene through illegitimate recombination with the T-cell receptor beta locus. Br J Haematol 144:546-51 (2009). WB ; Human . PubMed: 19055661