AB40777

重组Anti-IRS1+IRS2抗体[EP263Y]

Anti-IRS1+IRS2 antibody [EP263Y]

RabMAb
Recombinant
了解详情

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(30 Publications)

Rabbit Recombinant Monoclonal IRS1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples. Cited in 30 publications.

查看别名

Insulin receptor substrate 1, IRS-1, IRS1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma labelling IRS1 with unpurified ab40777.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling IRS1 with purified ab40777 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Flow Cytometry (Intracellular) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling IRS1 with unpurified ab40777 at 1/90 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling IRS1 with purified ab40777 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291 a mouse anti-tubulin (1/1000) and ab150120 an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/250) and secondary antibody ab150120 an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2 : ab7291 (1/1000) and secondary antibody ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).

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Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

All lanes:

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution

All lanes:

HeLa cell lysate at 10 µg

Observed band size: 170 kDa

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Lab

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/10000 dilution

All lanes:

HEK293 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 170 kDa

false

Lab

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777)

All lanes:

HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 170 kDa

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Supplier Data

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (AB40777)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. Exposure time : Lane 1, 2, 4, 5 , 6 : 1 second. Lane 3 : 80 seconds.

Lanes 1 - 3:

Western blot - Anti-IRS1+IRS2 antibody [EP263Y] (ab40777) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (<a href='/products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-epr20018-251-ab205606'>ab205606</a>) at 1/1000 dilution

Lanes 1 and 4:

HEK-293T transfected with an empty vector whole cell lysate at 20 µg

Lanes 2 and 5:

HEK-293T transfected with a human IRS1 expression vector containing a Myc/DDK tag whole cell lysate at 20 µg

Lanes 3 and 6:

HEK-293T transfected with a human IRS2 expression vector containing a Myc/DDK tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 170 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-IRS1+IRS2 antibody [EP263Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP263Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen used for this product shares 78% homology with IRS2. Cross-reactivity with this protein has not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The targets IRS1 and IRS2 also known as Insulin Receptor Substrate 1 and 2 are important adaptor proteins that play an important role in insulin signaling. IRS1 and IRS2 have molecular masses of approximately 180 kDa and 185 kDa respectively. These proteins are expressed in a wide range of tissues but show particularly high expression in insulin-responsive tissues such as muscle liver and adipose tissues. IRS1 and IRS2 function by interacting with activated insulin receptors allowing subsequent recruitment of downstream signaling molecules.

Biological function summary

IRS1 and IRS2 translate insulin receptor activation into metabolic and mitogenic effects. These proteins form signaling complexes that mediate the action of insulin and insulin-like growth factor 1 (IGF-1). IRS1 and IRS2 interact with several signaling molecules including PI3K and GRB2 which are essential for the activation of downstream pathways. Their role is essential in glucose homeostasis cell growth and differentiation. In some contexts IRS1 and IRS2 also participate in signaling pathways influenced by other growth factors.

Pathways

IRS1 and IRS2 have pivotal roles in the insulin signaling and IGF-1 signaling pathways. They facilitate signal transduction from the insulin and IGF-1 receptors impacting both metabolic and growth-related pathways. Within these pathways their interaction with PI3K highlights their importance in glucose uptake and utilization while their connection with SHC and ERK pathways emphasizes their involvement in cell growth and proliferation. The coordinated action of IRS1 and IRS2 helps maintain energy balance and cellular functions.

IRS1 and IRS2 have significant associations with type 2 diabetes and certain cancers. In type 2 diabetes alterations in IRS1 and IRS2 signaling can impair glucose metabolism leading to insulin resistance. Genetic variations or dysregulation in their function often contribute to diabetes susceptibility. Additionally IRS1 and IRS2 are linked to cancer as their pathways overlap with pathways that influence cell growth and survival. Increased signaling can enhance cell proliferation and resistance to apoptosis with relevant proteins like PI3K and AKT further involved in these processes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1

其他靶点

Insulin receptor substrate 2

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文献 (30)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:7197-7218 PubMed40585978

2025

Autophagy inhibitor-sensitized artificially activated neutrophils against hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Yang,Huang Yang,Zhengwei Mao,Weilin Wang,Yuan Ding

Aging 15:10291-10306 PubMed37812195

2023

polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Huahua Li,Jin Zheng,Yacen Wu,Hong Zhou,Suli Zeng,Quanqing Li

Heliyon 9:e16999 PubMed37408901

2023

LINC00638 promotes the progression of non-small cell lung cancer by regulating the miR-541-3p/IRS1/PI3K/Akt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Zhang,Yanhua Mou,Hui Li,Hui Shen,Jun Song,Qingfeng Li

Cancers 14: PubMed36551732

2022

Targeting IRS-1/2 in Uveal Melanoma Inhibits In Vitro Cell Growth, Survival and Migration, and In Vivo Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Chandrani Chattopadhyay,Rajat Bhattacharya,Jason Roszik,Fatima S Khan,Gabrielle A Wells,Hugo Villanueva,Yong Qin,Rishav Bhattacharya,Sapna P Patel,Elizabeth A Grimm

Clinical and experimental pharmacology & physiology 49:1307-1318 PubMed35986631

2022

Î²-Carotene regulates glucose transport and insulin resistance in gestational diabetes mellitus by increasing the expression of SHBG.

Applications

Unspecified application

Species

Unspecified reactive species

Xinli Liu,Nan Wang,Zhou Gao

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 55:e11741 PubMed35976267

2022

High expression of P4HA3 in obesity: a potential therapeutic target for type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Langen Zhuang,Can Li,Xiaolei Hu,Qingqing Yang,Xiaoyan Pei,Guoxi Jin

Bioengineered 13:2028-2043 PubMed35030964

2022

Long noncoding RNA ADIRF antisense RNA 1 upregulates insulin receptor substrate 1 to decrease the aggressiveness of osteosarcoma by sponging microRNA-761.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Xu,Yinling Tan,Fengxia Xu,Yong Zhang

iScience 24:102724 PubMed34337357

2021

Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling.

Applications

Unspecified application

Species

Unspecified reactive species