Anti-IRS1 (phospho Y896)抗体(ab4873)
Key features and details
- Rabbit polyclonal to IRS1 (phospho Y896)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IRS1 (phospho Y896)抗体
参阅全部 IRS1 一抗 -
描述
兔多克隆抗体to IRS1 (phospho Y896) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to IRS1 (phospho Y896).
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阳性对照
- Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate 1 (IRS 1). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/10 - 1/100.
|
| 说明 |
|---|
|
IHC-P
1/10 - 1/100. |
靶标
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功能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
疾病相关
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
序列相似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻译后修饰
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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数据库链接
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
别名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho Y896) antibody (ab4873)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling IRS1 with ab4873 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature and washed with ddH2O and PBS. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab4873 被引用在 4 文献中.
- Li H et al. Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy. Aging (Albany NY) 15:10291-10306 (2023). PubMed: 37812195
- Tan J & Guo L Swimming intervention alleviates insulin resistance and chronic inflammation in metabolic syndrome. Exp Ther Med 17:57-62 (2019). PubMed: 30651765
- Wang Y et al. Silencing of SAA1 inhibits palmitate- or high-fat diet induced insulin resistance through suppression of the NF-?B pathway. Mol Med 25:17 (2019). PubMed: 31060494
- Stuart CA et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). PubMed: 25472611