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Anti-IRS1 (phospho S616)抗体(ab4776)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Anti-IRS1 (phospho S616) antibody (ab4776)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)

Key features and details

  • Rabbit polyclonal to IRS1 (phospho S616)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-IRS1 (phospho S616)抗体
    参阅全部 IRS1 一抗
  • 描述

    兔多克隆抗体to IRS1 (phospho S616)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to IRS1 (phospho S616).

  • 阳性对照

    • WB: HEK-293T cells. IHC-P: Human breast carcinoma tissue, mouse skeletal muscle tissue.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA, 50% Glycerol

    BSA is IgG and protease free
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate 1 (IRS 1).
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

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相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IRS1 protein (ab70538)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4776于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 165 kDa.
说明
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 165 kDa.

靶标

  • 功能

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Target information above from: UniProt accession P35568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3667 Human
    • Entrez Gene: 16367 Mouse
    • Entrez Gene: 25467 Rat
    • Omim: 147545 Human
    • SwissProt: P35568 Human
    • SwissProt: P35569 Mouse
    • SwissProt: P35570 Rat
    • Unigene: 471508 Human
    • Unigene: 4952 Mouse
    • Unigene: 10476 Rat
    see all
  • 别名

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

图片

  • Western blot - Anti-IRS1 (phospho S616) antibody (ab4776)
    Western blot - Anti-IRS1 (phospho S616) antibody (ab4776)
    Extracts of 293T cells transfected with wild-type human IRS1 and treated with 100 ng/mL TPA for 30 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)

    Immunohistochemistry analysis of IRS1 (phospho S616) showing staining in the nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4776 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S616) antibody (ab4776)

    Immunohistochemistry analysis of IRS1 (phospho S616) showing staining in the nucleus of paraffin-embedded mouse skeletal muscle tissue (right) compared to a negative control without primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4776 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

实验方案

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab4776?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4776 被引用在 1 文献中.

  • Kawakami A  et al. Apolipoprotein CIII links hyperlipidemia with vascular endothelial cell dysfunction. Circulation 118:731-42 (2008). PubMed: 18663085

客户评价及客户问答

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Question

The S616 is this numbering in human or rat or another species. The numbering is slightly different depending upon species. Thank you for any help you might be able to give.

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Abcam community

Verified customer

Asked on Mar 09 2005

Answer

Thank you for your enquiry and patience. I contacted the originator of this antibody to see if they could provide clarification regarding this. Phospho-IRS-1 (Ser616) in human corresponds to Ser612 of mouse IRS-1. Also, the human, rat and mouse sequences are 100% homologous (identical, even) for this immunogenic sequence, but we can't confirm that the antibody will recognize all 3 species, since we've not tested them (recognition is expected, but not tested/confirmed). If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Mar 16 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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