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Anti-IRS1 (phospho S307)抗体(ab5599)

  • Datasheet
  • SDS
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Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)

Key features and details

  • Rabbit polyclonal to IRS1 (phospho S307)
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-IRS1 (phospho S307)抗体
    参阅全部 IRS1 一抗
  • 描述

    兔多克隆抗体to IRS1 (phospho S307)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, African green monkey
  • 免疫原

    Synthetic peptide corresponding to Human IRS1 aa 300-400 (phospho S307).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysate; NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysates. ICC: T-47D cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
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相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human IRS1 protein (ab70538)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5599于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.
说明
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ICC
Use a concentration of 1 - 2 µg/ml.

靶标

  • 功能

    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 疾病相关

    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 序列相似性

    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻译后修饰

    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Target information above from: UniProt accession P35568 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3667 Human
    • Entrez Gene: 16367 Mouse
    • Entrez Gene: 25467 Rat
    • Omim: 147545 Human
    • SwissProt: P35568 Human
    • SwissProt: P35569 Mouse
    • SwissProt: P35570 Rat
    • Unigene: 471508 Human
    • Unigene: 4952 Mouse
    • Unigene: 10476 Rat
    see all
  • 别名

    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

图片

  • Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
    Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)

    Immunocytochemistry analysis of 4% paraformaldehyde-fixed  0.25% Triton™ X-100 permeabilized T-47D cells (Human ductal breast epithelial tumor cell line) staining IRS1 with ab5599 at 1 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control uses no primary antibody. 

  • Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
    All lanes : Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/ml

    Lane 1 : Jurkat whole cell lysates
    Lane 2 : Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysates
    Lane 4 : NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 131 kDa
    Observed band size: 131 kDa



    Blocking buffer: 5% skimmed milk

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (10)

发表研究结果有使用 ab5599?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5599 被引用在 10 文献中.

  • Pan X  et al. Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells. Mol Med Rep 28:N/A (2023). PubMed: 37594055
  • Jiang A  et al. Free Fatty Acid Impairs Myogenic Differentiation through the AMPKα-MicroRNA 206 Pathway. Mol Cell Biol 42:e0032721 (2022). PubMed: 34694913
  • Shi YL  et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
  • Oswal N  et al. Host Metabolic Changes during Mycobacterium Tuberculosis Infection Cause Insulin Resistance in Adult Mice. J Clin Med 11:N/A (2022). PubMed: 35329973
  • Li L  et al. Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice. Food Funct 11:8743-8756 (2020). PubMed: 32955050
  • Yun C  et al. Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway. Exp Ther Med 19:923-930 (2020). PubMed: 32010253
  • Velickovic N  et al. Modulation of hepatic inflammation and energy-sensing pathways in the rat liver by high-fructose diet and chronic stress. Eur J Nutr 58:1829-1845 (2019). PubMed: 29845385
  • Ma L  et al. Intermittent Hypoxia Composite Abnormal Glucose Metabolism-Mediated Atherosclerosis In Vitro and In Vivo: The Role of SREBP-1. Oxid Med Cell Longev 2019:4862760 (2019). PubMed: 30863480
  • Chang RC  et al. Programmed increases in LXRa induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Mol Metab 30:161-172 (2019). PubMed: 31767168
  • Garabadu D & Krishnamurthy S Diazepam potentiates the antidiabetic, antistress and anxiolytic activities of metformin in type-2 diabetes mellitus with cooccurring stress in experimental animals. Biomed Res Int 2014:693074 (2014). WB ; Rat . PubMed: 24995322

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