Anti-IRS1 (phospho S307)抗体(ab1194)
Key features and details
- Rabbit polyclonal to IRS1 (phospho S307)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IRS1 (phospho S307)抗体
参阅全部 IRS1 一抗 -
描述
兔多克隆抗体to IRS1 (phospho S307) -
宿主
Rabbit -
特异性
Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig
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免疫原
Synthetic peptide corresponding to Human IRS1 aa 250-350 (phospho S307) conjugated to keyhole limpet haemocyanin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
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| 说明 |
|---|
|
WB
1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa). |
靶标
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功能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
疾病相关
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
序列相似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻译后修饰
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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数据库链接
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
别名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
图片
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Western blot - Anti-IRS1 (phospho S307) antibody (ab1194)All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution
Lane 1 : 293 cell lysate
Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
The 180kDa band is believed to correspond to phospho S307 IRS1. Blocking with the immunising peptide removes the 180kDa band (data not shown). A 4-20% Tris-Glycine gel was used, with blocking with 5% goat serum and 0.5% BLOTTO in PBS.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab1194 被引用在 8 文献中.
- Tian H et al. Effect of Dexmedetomidine-Mediated Insulin-Like Growth Factor 2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer. Med Sci Monit 25:4655-4664 (2019). PubMed: 31230061
- Liu SX et al. Exercise Reduces Insulin Resistance in Type 2 Diabetes Mellitus via Mediating the lncRNA MALAT1/MicroRNA-382-3p/Resistin Axis. Mol Ther Nucleic Acids 18:34-44 (2019). PubMed: 31479923
- Gallo JRB et al. Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery. Plast Reconstr Surg Glob Open 7:e2339 (2019). PubMed: 31592376
- Prabakaran AD et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). PubMed: 29434026
- Jiang J et al. Oncogenic activity of insulin in the development of non-small cell lung carcinoma. Oncol Lett 15:447-452 (2018). PubMed: 29387228
- Vidal-Quadras M et al. Endocytic turnover of Rab8 controls cell polarization. J Cell Sci 130:1147-1157 (2017). PubMed: 28137756
- Ma SB et al. Epigallocatechin-3-gallate ameliorates insulin resistance in hepatocytes. Mol Med Rep 15:3803-3809 (2017). PubMed: 28393248
- Stuart CA et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). PubMed: 25472611