重组Anti-IRS1抗体[EP260Y] (ab46800)
![Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)](https://www.abcam.cn/ps/products/46/ab46800/Images/ab46800-496881-anti-irs1-antibody-ep260y-western-blot-human.jpg "Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP260Y] to IRS1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-IRS1抗体[EP260Y]
参阅全部 IRS1 一抗 -
描述
兔单克隆抗体[EP260Y] to IRS1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: His-tagged human IRS1 recombinant protein (aa801-1020)
-
常规说明
This antibody was re-validated and was confirmed to recognize total IRS1. The name was changed from Anti-IRS1 (phospho Y896) antibody [EP260Y] to Anti-IRS1 antibody [EP260Y] on May 1st, 2018.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP260Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 27 kDa.
|
| 说明 |
|---|
|
WB
1/10000. Predicted molecular weight: 27 kDa. |
靶标
-
功能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
疾病相关
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
序列相似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻译后修饰
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
-
数据库链接
- Entrez Gene: 3667 Human
- Omim: 147545 Human
- SwissProt: P35568 Human
- Unigene: 471508 Human
-
别名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
图片
-
Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)Anti-IRS1 antibody [EP260Y] (ab46800) at 1/10000 dilution + His-tagged human IRS1 recombinant protein (aa801-1020), 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary and secondary antibodies incubated for 1hr at room temperature.
-
![Anti-IRS1 antibody [EP260Y] (ab46800)](https://www.abcam.cn/ps/products/46/ab46800/Images/ab46800-3-benefits-of-recombinant-antibodies.png)
Anti-IRS1 antibody [EP260Y] (ab46800)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab46800 被引用在 16 文献中.
- Zhao J et al. CircCCDC91 regulates chicken skeletal muscle development by sponging miR-15 family via activating IGF1-PI3K/AKT signaling pathway. Poult Sci 101:101803 (2022). PubMed: 35334442
- Peng L et al. Amelioration of Hippocampal Insulin Resistance Reduces Tau Hyperphosphorylation and Cognitive Decline Induced by Isoflurane in Mice. Front Aging Neurosci 13:686506 (2021). PubMed: 34512303
- Li L et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. J Cell Mol Med 25:2976-2993 (2021). PubMed: 33591626
- Rivera P et al. Perinatal free-choice of a high-calorie low-protein diet affects leptin signaling through IRS1 and AMPK dephosphorylation in the hypothalami of female rat offspring in adulthood. Acta Physiol (Oxf) 226:e13244 (2019). PubMed: 30589509
- Zhou D et al. Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression. Exp Mol Med 50:157 (2018). PubMed: 30510243