AB46800

Anti-IRS1 抗体 [EP260Y]

Anti-IRS1 antibody [EP260Y]

RabMAb
Recombinant
20ul selling size
了解详情

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(21 Publications)

Rabbit Recombinant Monoclonal IRS1 antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications.

查看别名

Insulin receptor substrate 1, IRS-1, IRS1

1 Images

Supplier Data

Western blot - Anti-IRS1 antibody [EP260Y] (AB46800)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Primary and secondary antibodies incubated for 1hr at room temperature.

Predicted band size of IRS1 recombinant protein fragment is 27 kDa.

All lanes:

Western blot - Anti-IRS1 antibody [EP260Y] (ab46800) at 1/10000 dilution

All lanes:

His-tagged human IRS1 recombinant protein (aa801-1020) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

false

Exposure time: 1s

不同偶联物与剂型 (1)

Carrier free

Anti-IRS1 antibody [EP260Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP260Y

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

This antibody was re-validated and was confirmed to recognize total IRS1. The name was changed from Anti-IRS1 (phospho Y896) antibody [EP260Y] to Anti-IRS1 antibody [EP260Y] on May 1st, 2018.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.

Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1

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文献 (21)

Recent publications for all applications. Explore the full list and refine your search

Biology of sex differences 16:43 PubMed40524248

2025

Gestational saccharin consumption disrupts gut-brain axis glucose homeostasis control in adolescent offspring rats in a sex-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Beatriz Pacheco-Sánchez,Sonia Melgar-Locatelli,Raquel López-Merchán,María José Benítez-Marín,Marta Blasco-Alonso,Ernesto González-Mesa,Rubén Tovar,Pablo Rubio,Juan Suárez,Carlos Sanjuan,Fernando Rodríguez de Fonseca,Francisco Alén,Marialuisa de Ceglia,Patricia Rivera

Open medicine (Warsaw, Poland) 18:20230855 PubMed38045856

2023

Cimifugin inhibits adipogenesis and TNF-α-induced insulin resistance in 3T3-L1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Deng,Zhenmin Liu,Siqi Han

Molecular nutrition & food research 68:e2200842 PubMed37990402

2023

Goat Milk Improves Glucose Metabolism in Type 2 Diabetic Mice and Protects Pancreatic β-Cell Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Chen,Zhifei Zhang,Huiming Niu,Xinmiao Tian,Huibin Tian,Weiwei Yao,Huanshan He,Huaiping Shi,Cong Li,Jun Luo

Theriogenology 211:241-247 PubMed37677868

2023

Association of glucocorticoid receptor expression with key members of the insulin signaling pathway and heat shock proteins in the bovine ovary.

Applications

Unspecified application

Species

Unspecified reactive species

F M Rodríguez,E Huber,M L Cattaneo Moreyra,A N Amweg,U S Notaro,S Recce,N Ormaechea,H H Ortega,N R Salvetti,F Rey

Poultry science 101:101803 PubMed35334442

2022

CircCCDC91 regulates chicken skeletal muscle development by sponging miR-15 family via activating IGF1-PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Xiyu Zhao,Xiaoxu Shen,Yun Zhang,Yao Zhang,Lin Ye,Diyan Li,Qing Zhu,Huadong Yin

Frontiers in aging neuroscience 13:686506 PubMed34512303

2021

Amelioration of Hippocampal Insulin Resistance Reduces Tau Hyperphosphorylation and Cognitive Decline Induced by Isoflurane in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liangyu Peng,Xin Fang,Fangxia Xu,Shuai Liu,Yue Qian,Xiangdan Gong,Xin Zhao,Zhengliang Ma,Tianjiao Xia,Xiaoping Gu

Journal of cellular and molecular medicine 25:2976-2993 PubMed33591626

2021

Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhao Li,Xin Zeng,Zhenzhen Liu,Xuanming Chen,Lan Li,Ruixi Luo,Xiaohong Liu,Jie Zhang,Jingping Liu,Yanrong Lu,Jingqiu Cheng,Younan Chen

Journal of molecular endocrinology 64:181-193 PubMed31990656

2020

PGRN exerts inflammatory effects via SIRT1-NF-κB in adipose insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Huixia Li,Zhuanmin Zhang,Dongxu Feng,Lin Xu,Fang Li,Jiali Liu,Xinxin Jin,Zhuang Qian,Xiaomin Kang,Hongzhi Sun

Acta physiologica (Oxford, England) 226:e13244 PubMed30589509

2019

Perinatal free-choice of a high-calorie low-protein diet affects leptin signaling through IRS1 and AMPK dephosphorylation in the hypothalami of female rat offspring in adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Rivera,María T Ramírez-López,Antonio Vargas,Juan Decara,Mariam Vázquez,Rocío Arco,Raquel Gómez de Heras,Jesús Argente,Fernando Rodríguez de Fonseca,Julie A Chowen,Juan Suárez

Experimental & molecular medicine 50:1-12 PubMed30510243

2018

Sodium butyrate reduces high-fat diet-induced non-alcoholic steatohepatitis through upregulation of hepatic GLP-1R expression.

Applications

Unspecified application

Species

Unspecified reactive species

Da Zhou,Yuan-Wen Chen,Ze-Hua Zhao,Rui-Xu Yang,Feng-Zhi Xin,Xiao-Lin Liu,Qin Pan,Huiping Zhou,Jian-Gao Fan

View all publications

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