重组Anti-IRR抗体[EP327Y] (ab40782)
![Western blot - Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.cn/ps/products/40/ab40782/Images/ab40782-53953-anti-irr-antibody-ep327y-western-blot.jpg "Western blot - Anti-IRR antibody [EP327Y] (ab40782)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP327Y] to IRR
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-IRR抗体[EP327Y]
参阅全部 IRR 一抗 -
描述
兔单克隆抗体[EP327Y] to IRR -
宿主
Rabbit -
特异性
ab40782 recognises insulin receptor related receptor (IRR). -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human IRR aa 1-100 (N terminal). The exact sequence is proprietary.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP327Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40782于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
This receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3645 Human
- Entrez Gene: 23920 Mouse
- Entrez Gene: 60663 Rat
- Omim: 147671 Human
- SwissProt: P14616 Human
- SwissProt: Q9WTL4 Mouse
- SwissProt: Q64716 Rat
- Unigene: 248138 Human
see all -
别名
- INSRR antibody
- INSRR_HUMAN antibody
- Insulin Receptor R antibody
see all
图片
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Western blot - Anti-IRR antibody [EP327Y] (ab40782)All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SHSY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 80, 144 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.cn/ps/products/40/ab40782/Images/ab40782-53952-anti-irr-antibody-ep327y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] (ab40782)Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.cn/ps/products/40/ab40782/Images/ab40782-3-benefits-of-recombinant-antibodies.png)
Anti-IRR antibody [EP327Y] (ab40782)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab40782 被引用在 3 文献中.
- Liu H et al. Magnesium supplementation enhances insulin sensitivity and decreases insulin resistance in diabetic rats. Iran J Basic Med Sci 23:990-998 (2020). PubMed: 32952944
- Wang L et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). PubMed: 28710412
- Li Y et al. LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and kidney malformations in Cenani-Lenz syndrome. Am J Hum Genet 86:696-706 (2010). WB ; Human . PubMed: 20381006