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重组RabMAb

重组Anti-Iroquois homeobox蛋白3/IRX3抗体[EPR22709-52] - BSA and Azide free (ab274328)

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Immunocytochemistry/ Immunofluorescence - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Flow Cytometry (Intracellular) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
  • Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22709-52] to Iroquois homeobox protein 3/IRX3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Iroquois homeobox蛋白3/IRX3抗体[EPR22709-52] - BSA and Azide free
    参阅全部 Iroquois homeobox protein 3/IRX3 一抗

  • 描述

    兔单克隆抗体[EPR22709-52] to Iroquois homeobox蛋白3/IRX3 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
    不适用于: IHC-Fr or IP

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse embryo, E14.5 lung and E17 lung tissue lysates; Rat E17 brain (primary culture) tissue lysate. IHC-P: Mouse E14.5 heart and E14.5 lung tissue; Rat E14.5 heart tissue. ICC/IF: HEK-293T cells (transfected with Flag-tagged mouse IRX3 expression vector). Flow Cyt (intra): HEK-293T cells (transfected with Flag-tagged mouse IRX3 expression vector).

  • 常规说明

    ab274328 is the carrier-free version of ab242133.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR22709-52

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274328于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 52 kDa).
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 52 kDa).
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-Fr or IP.

靶标

  • 功能

    Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.

  • 序列相似性

    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI894186 antibody
      • Homeodomain protein IRXB1 antibody
      • Iroquois class homeodomain protein IRX 1 antibody
      • Iroquois homeobox 3 antibody
      • Iroquois homeobox protein 3 antibody
      • Iroquois-class homeodomain protein irx-3 antibody
      • IRX 1 antibody
      • irx3 antibody
      • IRX3_HUMAN antibody
      • IRXB1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Immunocytochemistry/ Immunofluorescence - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells (transfected with Flag-tagged mouse IRX3 expression vector) labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with Flag-tagged IRX3 expression vector. is observed. Anti-FLAG mouse monoclonal antibody was used at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse E14.5 lung tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lung of mouse E14.5 (PMID: 11520674 ). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Flow Cytometry (Intracellular) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Flow Cytometry (Intracellular) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

      This data was developed using ab242133, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with Flag-tagged mouse IRX3 expression vector) cells labelling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

      Lane 1 : Mouse E17 embryo tissue lysate
      Lane 2 : Rat E17 brain (primary culture) tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 52 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This blot was developed using a higher sensitivity ECL substrate.

      Exposure time: 125 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of rat E14.5. The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] (ab242133) at 1/1000 dilution

      Lane 1 : Mouse embryo tissue lysate
      Lane 2 : Mouse E14.5 lung tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 52 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This blot was developed using a higher sensitivity ECL substrate.

      Exposure time: 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

      This data was developed using ab242133, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse E14.5 heart tissue labeling Iroquois homeobox protein 3/IRX3 with ab242133 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on heart of mouse E14.5 (PMID: 15342473, PMID: 11520674). The section was incubated with ab242133 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)
      Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR22709-52] - BSA and Azide free (ab274328)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab274328 尚未被引用在任何文献中。

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