重组Anti-IRF7抗体[EPR23509-21] - BSA and Azide free (ab272225)
![Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)](https://www.abcam.cn/ps/products/272/ab272225/Images/ab272225-387956-anti-irf7-antibody-epr23509-21-immunoprecipitation-human.jpg "Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23509-21] to IRF7 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRF7抗体[EPR23509-21] - BSA and Azide free
参阅全部 IRF7 一抗 -
描述
兔单克隆抗体[EPR23509-21] to IRF7 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ChIP,Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate. IP: Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 µM MG-132 for another 6 hours, whole cell lysate.
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常规说明
ab272225 is the carrier-free version of ab238137.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23509-21 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 65, 45 kDa (predicted molecular weight: 54 kDa).
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 65, 45 kDa (predicted molecular weight: 54 kDa). |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability. -
组织特异性
Expressed predominantly in spleen, thymus and peripheral blood leukocytes. -
序列相似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻译后修饰
In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation. -
细胞定位
Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3665 Human
- Omim: 605047 Human
- SwissProt: Q92985 Human
- Unigene: 166120 Human
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别名
- IMD39 antibody
- Interferon regulatory factor 7 antibody
- Interferon regulatory factor 7H antibody
see all
图片
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Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
IRF7 was immunoprecipitated from 0.35 mg of Jurkat (treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours) whole cell lysate with ab238137 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238137 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat (treated as above) whole cell lysate 10 μg (Input).
Lane 2: ab238137 IP in Jurkat (treated as above) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238137 in Jurkat (treated as above) whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 seconds.65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID: 23175366).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab238137).
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![Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)](https://www.abcam.cn/ps/products/272/ab272225/Images/ab272225-2-benefits-of-recombinant-antibodies.png)
Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free (ab272225)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab272225 尚未被引用在任何文献中。
