AB238137

重组Anti-IRF7抗体[EPR23509-21]

Anti-IRF7 antibody [EPR23509-21]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal IRF7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications.

查看别名

Interferon regulatory factor 7, IRF-7, IRF7

2 Images

Lab

Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] (AB238137)

IRF7 was immunoprecipitated from 0.35 mg of Jurkat (treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours) whole cell lysate with ab238137 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab238137 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Jurkat (treated as above) whole cell lysate 10 μg (Input).
Lane 2 : ab238137 IP in Jurkat (treated as above) whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab238137 in Jurkat (treated as above) whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 84 seconds.

65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID : 23175366).

All lanes:

Immunoprecipitation - Anti-IRF7 antibody [EPR23509-21] (ab238137)

Predicted band size: 54 kDa

Observed band size: 45 kDa,65 kDa

false

Lab

Western blot - Anti-IRF7 antibody [EPR23509-21] (AB238137)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 37 seconds.

The expression of IRF7 is induced by IFN alpha in Jurkat (PMID : 9786932).

65-kDa full length IRF7 and 45-kDa C-terminal cleaved IRF7 are observed (PMID : 23175366).

All lanes:

Western blot - Anti-IRF7 antibody [EPR23509-21] (ab238137) at 1/5000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

Jurkat treated with 100 nM IFN alpha for 18 hours, then added 10 μM MG-132 for another 6 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa,65 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-IRF7 antibody [EPR23509-21] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23509-21

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The transcription factor IRF7 also known as Interferon Regulatory Factor 7 plays a significant role in the regulation of type I interferon genes. This protein acts as an important modulator of the immune response specifically during viral infections. The molecular weight of IRF7 is approximately 54 kDa. It is predominantly expressed in lymphoid tissues such as the spleen and thymus and is also found in other immune cells like B cells and dendritic cells. The expression of IRF7 is induced by pathogen-associated molecular patterns which stimulate the activation of immune signaling pathways.

Biological function summary

IRF7 activates and modulates the expression of type I interferons and other cytokines important for antiviral defense. This protein can form part of a larger complex with other IRF family members to enhance its effect on gene transcription. IRF7 operates through its interaction with transcriptional coactivators allowing it to execute its function as a transcriptional activator reinforcing the immune system's response to infectious agents.

Pathways

IRF7 plays a critical role in the Toll-like receptor (TLR) signaling pathway and retinoic acid-inducible gene I (RIG-I) pathway. It directly interacts with MyD88 and IRAK1 proteins linking it to signal transduction pathways that lead to interferon-beta production. The activation of IRF7 in these pathways also strengthens the body's defense mechanism by enhancing the transcription of antiviral genes forming a robust antibacterial and antiviral environment within the host.

IRF7's function is implicated in autoimmune diseases and specific cancers. For autoimmune conditions like systemic lupus erythematosus (SLE) dysregulated IRF7 activity is observed leading to an aberrant interferon response. It connects with proteins such as IRF5 which has its expressions elevated in affected patients. In relation to cancer IRF7 can impact tumor progression particularly in breast cancer through modulation of immune surveillance functions. Its interaction with interferon pathways becomes a double-edged sword affecting tumor immunity and progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 28342865, PubMed : 28768858). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 17574024, PubMed : 32972995). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner (By similarity). Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages (By similarity) (PubMed : 11073981, PubMed : 12374802, PubMed : 15361868, PubMed : 17404045).

See full target information IRF7

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:116166 PubMed40833856

2025

Alternatively spliced isoforms of IRF7 differentially regulate interferon expression to tune response to viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Asmita Panthi,Max B Ferretti,Olivia Howard,Swechha Mainali Pokharel,Rhiannon McCracken,Simon Boudreault,Mathieu Quesnel-Vallieres,Qin Li,Sara Cherry,Kristen W Lynch

Clinical and translational medicine 14:e1799 PubMed39118300

2024

Single-cell sequencing combined with spatial transcriptomics reveals that the IRF7 gene in M1 macrophages inhibits the occurrence of pancreatic cancer by regulating lipid metabolism-related mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhan,Yanli Zou,Zheng Han,XiaoRong Tian,Mengge Chen,Jiaxi Liu,Xiulin Yang,Qingxi Zhu,Meng Liu,Wei Chen,Mingtao Chen,Xiaodong Huang,Jie Tan,Weijie Liu,Xia Tian

Cancer communications (London, England) 44:408-432 PubMed38407943

2024

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Tang,Huan Zhang,Fen Zhou,Qiuzhe Wei,Mengyi Du,Jianghua Wu,Chenggong Li,Wenjing Luo,Jie Zhou,Xindi Wang,Zhaozhao Chen,Yinqiang Zhang,Zhongpei Huang,Zhuolin Wu,Yuxi Wen,Huiwen Jiang,Danying Liao,Haiming Kou,Wei Xiong,Heng Mei,Yu Hu

Poultry science 103:103274 PubMed38043405

2023

Chlorogenic acid protects against intestinal inflammation and injury by inactivating the mtDNA-cGAS-STING signaling pathway in broilers under necrotic enteritis challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Lv,Peng Chen,Yang Wang,Lianbin Xu,Kai Zhang,Jinshan Zhao,Huawei Liu

Viruses 14: PubMed36366509

2022

H1N1 Influenza A Virus Protein NS2 Inhibits Innate Immune Response by Targeting IRF7.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Minxuan Liu,Jiaxin Huang,Qiaoying Zeng,Qiyun Zhu,Shuai Xu,Hualan Chen

Experimental dermatology 31:1165-1176 PubMed35332586

2022

Ultraviolet light induces HERV expression to activate RIG-I signalling pathway in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Meiling Zheng,Yaqin Yu,Jiali Wu,Qiqi Kuang,Zhi Hu,Lianlian Ouyang,Shuang Lu,Ming Zhao

Science advances 7: PubMed33863728

2021

SRSF1 serves as a critical posttranscriptional regulator at the late stage of thymocyte development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihong Qi,Fang Wang,Guotao Yu,Di Wang,Yingpeng Yao,Menghao You,Jingjing Liu,Juanjuan Liu,Zhen Sun,Ce Ji,Yuanchao Xue,Shuyang Yu

View all publications

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重组Anti-IRF7抗体[EPR23509-21]