Anti-IRF4抗体[MUM1p] (ab12501)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501)](https://www.abcam.cn/ps/products/12/ab12501/Images/ab12501-20058-anti-mum1-antibody-mum1p-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501)")
Key features and details
- Mouse monoclonal [MUM1p] to IRF4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IRF4抗体[MUM1p]
参阅全部 IRF4 一抗 -
描述
小鼠单克隆抗体[MUM1p] to IRF4 -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IRF4.
Database link: Q15306 -
阳性对照
- Tonsil.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
MUM1p -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
| 说明 |
|---|
应用说明
IHC-P: 1/10 - 1/25. Perform heat mediated antigen retrieval with 1 mM EDTA buffer, pH 9.0 before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes. -
组织特异性
Lymphoid cells. -
疾病相关
Multiple myeloma -
序列相似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻译后修饰
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3662 Human
- SwissProt: Q15306 Human
- Unigene: 401013 Human
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别名
- Interferon regulatory factor 4 antibody
- IRF 4 antibody
- IRF-4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501)IHC image of ab12501 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab12501 被引用在 5 文献中.
- Tessier-Cloutier B et al. Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival. Lupus Sci Med 6:e000324 (2019). PubMed: 31205728
- Kinoshita M et al. Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis. Neuro Oncol 16:728-34 (2014). Human . PubMed: 24497406
- Aguilera NS et al. Activation-induced cytidine deaminase expression in diffuse large B-cell lymphoma with a paracortical growth pattern: a lymphoma of possible interfollicular large B-cell origin. Arch Pathol Lab Med 134:449-56 (2010). IHC-P ; Human . PubMed: 20196672
- Fridberg M et al. Protein expression and cellular localization in two prognostic subgroups of diffuse large B-cell lymphoma: Higher expression of ZAP70 and PKC-beta II in the non-germinal center group and poor survival in patients deficient in nuclear PTEN. Leuk Lymphoma 48:2221-32 (2007). IHC-P ; Human . PubMed: 17926183
- Fukushima N et al. Clinico-pathological characteristics of p63 expression in B-cell lymphoma. Cancer Sci 97:1050-5 (2006). PubMed: 16918993