重组Anti-IRF4抗体[EPR5653] (ab124691)
![Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-8-anti-mum1-antibody-epr5653-immunoprecipitation-ramos-human.jpg "Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5653] to IRF4
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRF4抗体[EPR5653]
参阅全部 IRF4 一抗 -
描述
兔单克隆抗体[EPR5653] to IRF4 -
宿主
Rabbit -
特异性
Uniprot ID - Q15306 -
经测试应用
适用于: WB, IP, IHC-P, ChIPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Ramos, HuT 78 and IM 9 cell lysates; IHC-P: Human colon and tonsil tissues. IP: Ramos lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 4.18 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5653 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-IRF4 antibody [EPR5653] - BSA and Azide free (ab247962)
- PE Anti-IRF4 antibody [EPR5653] (ab318374)
- APC Anti-IRF4 antibody [EPR5653] (ab318477)
- Alexa Fluor® 488 Anti-IRF4 antibody [EPR5653] (ab318580)
- Alexa Fluor® 647 Anti-IRF4 antibody [EPR5653] (ab318683)
- Alexa Fluor® 594 Anti-IRF4 antibody [EPR5653] (ab318786)
- Alexa Fluor® 555 Anti-IRF4 antibody [EPR5653] (ab318886)
- Alexa Fluor® 750 Anti-IRF4 antibody [EPR5653] (ab320974)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124691于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
|
| IP |
1/10 - 1/100.
|
|
| IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| ChIP |
Use 5 µg for 25 µg of chromatin.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ChIP
Use 5 µg for 25 µg of chromatin. |
靶标
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功能
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes. -
组织特异性
Lymphoid cells. -
疾病相关
Multiple myeloma -
序列相似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻译后修饰
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3662 Human
- SwissProt: Q15306 Human
- Unigene: 401013 Human
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别名
- Interferon regulatory factor 4 antibody
- IRF 4 antibody
- IRF-4 antibody
see all
图片
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Immunoprecipitation - Anti-IRF4 antibody [EPR5653] (ab124691)
IRF4 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with 124691 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg
Lane 2: ab124691 IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124691 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-138463-anti-mum1-antibody-epr5653-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)ab124691, at 1/100, staining IRF4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![ChIP - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-482335-antimum1-antibody-epr5653-chip.jpg)
ChIP - Anti-IRF4 antibody [EPR5653] (ab124691)Chromatin was prepared from SK-MEL-28 cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab124691 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).ChIP was performed according to the literature (PMID: 24267888)
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![Western blot - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-138465-anti-mum1-antibody-epr5653-western-blot.jpg)
Western blot - Anti-IRF4 antibody [EPR5653] (ab124691)All lanes : Anti-IRF4 antibody [EPR5653] (ab124691) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : IM 9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-138464-anti-mum1-antibody-epr5653-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)ab124691, at 1/100, staining IRF4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-138462-anti-mum1-antibody-epr5653-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [EPR5653] (ab124691)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of dog spleen tissue, staining IRF4 with ab124691.
Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody. -
![OI-RD Scanning - Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-138461-anti-mum1-antibody-epr5653.jpg)
OI-RD Scanning - Anti-IRF4 antibody [EPR5653] (ab124691) -
![Anti-IRF4 antibody [EPR5653] (ab124691)](https://www.abcam.cn/ps/products/124/ab124691/Images/ab124691-7-benefits-of-recombinant-antibodies.png)
Anti-IRF4 antibody [EPR5653] (ab124691)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab124691 被引用在 3 文献中.
- Park KH et al. Extramedullary plasmacytoma of the oral cavity metastasising to both kidneys in a dog. Vet Med Sci 9:1053-1061 (2023). PubMed: 36748292
- Sun R et al. Prognostic significance of interferon regulating factor 4 in esophageal squamous cell carcinoma. Biochem Biophys Res Commun 506:685-691 (2018). PubMed: 30376993
- Vieyra-Garcia PA et al. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides. Clin Cancer Res 22:3328-39 (2016). IHC-P ; Human . PubMed: 26851186