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Anti-IRF3抗体[OTI4A1] - BSA and Azide free (ab273663)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
  • Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

Key features and details

  • Mouse monoclonal [OTI4A1] to IRF3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-IRF3抗体[OTI4A1] - BSA and Azide free
    参阅全部 IRF3 一抗

  • 描述

    小鼠单克隆抗体[OTI4A1] to IRF3 - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human IRF3 aa 27-169. NP_001184053 Produced in E.coli
    Database link: Q14653

  • 阳性对照

    • WB: IRF3 transfecredHEK-293T cell lysate. IHC-P: Human colon adenocarcinoma, ovary, pancreas, thyroid and endometrium tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273663于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000. Predicted molecular weight: 47 kDa.
IHC-P
1/150.

Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3minutes.
说明
WB
1/2000. Predicted molecular weight: 47 kDa.
IHC-P
1/150.

Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3minutes.

靶标

  • 功能

    Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

  • 组织特异性

    Expressed constitutively in a variety of tissues.

  • 序列相似性

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

  • 翻译后修饰

    Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
    Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
    ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

  • 细胞定位

    Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • IIAE7 antibody
      • Interferon regulatory factor 3 antibody
      • IRF 3 antibody
      • IRF-3 antibody
      • IRF3 antibody
      • IRF3_HUMAN antibody
      • MGC94729 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

      Paraffin embedded human colon adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

       

       

    • Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      All lanes : Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663) at 1/2000 dilution

      Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control
      Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IRF3

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 47 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

      Paraffin embedded human ovary tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

      Paraffin embedded human pancreas tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

      Paraffin embedded carcinoma of Human thyroid tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663)

      Paraffin embedded  human endometrium adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273663？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273663 尚未被引用在任何文献中。

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