AB273663

Anti-IRF3抗体[OTI4A1] - BSA and Azide free

Anti-IRF3 antibody [OTI4A1] - BSA and Azide free

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Mouse Monoclonal IRF3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IRF3 aa 1-200.

查看别名

Interferon regulatory factor 3, IRF-3, IRF3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

Paraffin embedded human ovary tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

Paraffin embedded human endometrium adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

Paraffin embedded carcinoma of Human thyroid tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

Paraffin embedded human colon adenocarcinoma tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

Paraffin embedded human pancreas tissue stained for IRF3 using ab273663 at 1/150 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

WB

Supplier Data

Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (AB273663)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, pH 7.3, 8% trehalose.

All lanes:

Western blot - Anti-IRF3 antibody [OTI4A1] - BSA and Azide free (ab273663) at 1/2000 dilution

Lane 1:

HEK-293T cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY IRF3 at 5 µg

Predicted band size: 47 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI4A1

亚型

IgG1

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

免疫原

Recombinant Fragment Protein within Human IRF3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3minutes.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from tissue culture supernatant.

存储溶液

pH: 7.3 Constituents: PBS

运输条件

Blue Ice

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IRF3 also known as Interferon Regulatory Factor 3 acts as an important transcription factor in the immune response. It has a molecular weight of approximately 47 kDa. The IRF3 protein is mainly expressed in the cytoplasm and nucleus of various cell types including immune cells such as macrophages and dendritic cells. The protein becomes activated through phosphorylation a process frequently identified in its phosphorylated form phospo-IRF3 or p-IRF3 which facilitates its role in immune function.

Biological function summary

IRF3 participates in the regulation of type I interferon (IFN) response a fundamental antiviral defense mechanism. IRF3 when phosphorylated forms a complex with CBP/p300 which then translocates to the nucleus to drive the expression of IFN-stimulated genes. This action strengthens the innate immune response and boosts the body's ability to counteract viral infections. Its activity and regulation are significant for maintaining a balanced immune response without excessive inflammation.

Pathways

IRF3 is involved in the Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling pathways both essential in pathogen recognition and response. Within these pathways IRF3 interacts with proteins such as MAVS and TBK1 to propagate immune signaling. The activation of IRF3 in these pathways results in the production of type I interferons and other cytokines orchestrating an effective antiviral response. These interactions highlight the protein's central role in mediating immune signaling cascades.

IRF3's malfunction or deregulation can contribute to autoimmune diseases and antiviral deficiencies. Conditions such as systemic lupus erythematosus (SLE) and chronic hepatitis B infection are linked to IRF3 activity. During autoimmune responses or viral persistence the aberrant activation of IRF3 can lead to inappropriate immune responses. The connection with proteins like STAT1 in these conditions highlights the complex network IRF3 engages in facilitating its impact on disease progression and immune dysregulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed : 22394562, PubMed : 24049179, PubMed : 25636800, PubMed : 27302953, PubMed : 31340999, PubMed : 36603579, PubMed : 8524823, PubMed : 39362857). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 11846977, PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 32972995, PubMed : 36603579, PubMed : 8524823). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed : 16846591, PubMed : 16979567, PubMed : 20049431, PubMed : 36603579). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed : 22394562, PubMed : 25636800, PubMed : 27302953, PubMed : 36603579). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed : 16154084, PubMed : 27302953, PubMed : 33440148, PubMed : 36603579). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed : 16846591). In response to Sendai virus infection, is recruited by TOMM70 : HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70 : HSP90AA1 : IRF3 : BAX inducing apoptosis (PubMed : 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information IRF3

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