Anti-IRF3抗体[IRF35I218] (ab50772)
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.cn/ps/products/50/ab50772/Images/ab50772-62462-anti-irf3-antibody-irf35i218-western-blot.jpg "Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)")
Key features and details
- Mouse monoclonal [IRF35I218] to IRF3
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IRF3抗体[IRF35I218]
参阅全部 IRF3 一抗 -
描述
小鼠单克隆抗体[IRF35I218] to IRF3 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: VAWVNKSRTR FRIPWKHGLR QDAQQEDFGI FQAWAEATGA YVPGRDKPDL PTWKRNFRSA LNRKEGLRLA EDRSKD, corresponding to amino acids 24-150 of Human IRF3
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阳性对照
- HEK293 whole cell lysate RIPA lysate of HeLa cells Hela cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.316% Tris HCl, 1% BSA, 1.16% Sodium chloride -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Concentrated and dialyzed culture medium (protein free medium) of hybridoma -
克隆
单克隆 -
克隆编号
IRF35I218 -
同种型
IgG1 -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50772于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/10.
|
|
| WB | (3) |
1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
ICC/IF
1/10. |
|
WB
1/50 - 1/100. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa). |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains. -
组织特异性
Expressed constitutively in a variety of tissues. -
序列相似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻译后修饰
Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. -
细胞定位
Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3661 Human
- Omim: 603734 Human
- SwissProt: Q14653 Human
- Unigene: 289052 Human
- Unigene: 75254 Human
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别名
- IIAE7 antibody
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
see all
图片
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Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg
Secondary
Anti mouse IgG at 1/2500 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.cn/ps/products/50/ab50772/Images/ab50772-210362-anti-irf3-antibody-irf35i218-flow-cytometry.jpg)
Flow Cytometry - Anti-IRF3 antibody [IRF35I218] (ab50772)Overlay histogram showing HeLa cells stained with ab50772 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50772, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.cn/ps/products/50/ab50772/Images/ab50772-62463-anti-irf3-antibody-irf35i218-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody [IRF35I218] (ab50772)Immunofluorescent staining of HeLa cells with a 1/10 dilution of ab50772 as primary antibody and AlexaFluor®488 conjugated Goat Anti-mouse IgG diluted 1/200 as the secondary antibody -
![Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)](https://www.abcam.cn/ps/products/50/ab50772/Images/ab50772-62461-anti-irf3-antibody-irf35i218-western-blot.jpg)
Western blot - Anti-IRF3 antibody [IRF35I218] (ab50772)Anti-IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg
Secondary
Anti-mouse IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
IRF3 antibody [IRF35I218] (ab50772) at 1/100 dilution + Ripa lysate of Hela cells at 500 µg Secondary Anti-mouse IgG at 1/1000 dilution
实验方案
数据表及文件
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Datasheet download
文献 (9)
ab50772 被引用在 9 文献中.
- Uhlorn BL et al. Attenuation of cGAS/STING activity during mitosis. Life Sci Alliance 3:N/A (2020). PubMed: 32661021
- Uhlorn BL et al. Vesicular trafficking permits evasion of cGAS/STING surveillance during initial human papillomavirus infection. PLoS Pathog 16:e1009028 (2020). PubMed: 33253291
- Yi L et al. Interferon regulatory factor 3 mediates Poly(I:C)-induced innate immune response and apoptosis in non-small cell lung cancer. Int J Oncol 52:1623-1632 (2018). PubMed: 29512705
- Wang CH et al. Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells. Mol Ther Methods Clin Dev 6:194-206 (2017). PubMed: 28831401
- Iqbal J et al. Histone H2B-IFI16 Recognition of Nuclear Herpesviral Genome Induces Cytoplasmic Interferon-ß Responses. PLoS Pathog 12:e1005967 (2016). PubMed: 27764250
- Brownell J et al. Direct, interferon-independent activation of the CXCL10 promoter by NF-?B and interferon regulatory factor 3 during hepatitis C virus infection. J Virol 88:1582-90 (2014). PubMed: 24257594
- Wang J et al. Morphine inhibits murine dendritic cell interleukin 23 production by modulating TLR2 and Nod2 signaling. J Biol Chem : (2011). ICC/IF ; Mouse . PubMed: 21245149
- Holm CK et al. TLR3 ligand polyinosinic:polycytidylic acid induces IL-17A and IL-21 synthesis in human Th cells. J Immunol 183:4422-31 (2009). WB ; Human . PubMed: 19748983
- Chen GY et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). ICC/IF ; Human . PubMed: 19656899
