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重组RabMAb

重组Anti-IREB2/IRP2 + Aconitase 1/ACO1抗体[EPR13854] (ab181153)

Submit a review Submit a question References (13)
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
  • Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
  • Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13854] to IREB2/IRP2 + Aconitase 1/ACO1
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-IREB2/IRP2 + Aconitase 1/ACO1抗体[EPR13854]
    参阅全部 IREB2/IRP2+Aconitase 1/ACO1 一抗

  • 描述

    兔单克隆抗体[EPR13854] to IREB2/IRP2 + Aconitase 1/ACO1

  • 宿主

    Rabbit

  • 特异性

    The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

  • 经测试应用

    适用于: WB, Flow Cyt (Intra)more details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: A549, Jurkat, Human fetal kidney or Human fetal liver lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab181153于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 105 kDa).
Flow Cyt (Intra)
1/30.
说明
WB
1/1000 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 105 kDa).
Flow Cyt (Intra)
1/30.
应用说明
Is unsuitable for ICC/IF.

靶标

图片

  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

    Lane 1 : Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg
    Lane 3 : Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 105 kDa
    Observed band size: 106 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM /TBST

  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (unpurified)

    Lane 1 : A549 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

    Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

     

     

  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    All lanes : Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution (purified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST 

  • Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/5000 dilution (unpurified) + Human fetal liver cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 105 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
    Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)

文献 (13)

发表研究结果有使用 ab181153？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181153 被引用在 13 文献中.

  • Zhong X & Chen R Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence. Methods Mol Biol 2712:211-222 (2023). PubMed: 37578709
  • Chang B  et al. Cox4i2 Triggers an Increase in Reactive Oxygen Species, Leading to Ferroptosis and Apoptosis in HHV7 Infected Schwann Cells. Front Mol Biosci 8:660072 (2021). PubMed: 34026834
  • Abdul Y  et al. Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis. Transl Stroke Res 12:615-630 (2021). PubMed: 32875455
  • Dubreuil MM  et al. Systematic Identification of Regulators of Oxidative Stress Reveals Non-canonical Roles for Peroxisomal Import and the Pentose Phosphate Pathway. Cell Rep 30:1417-1433.e7 (2020). PubMed: 32023459
  • Du J  et al. Identification of Frataxin as a regulator of ferroptosis. Redox Biol 32:101483 (2020). PubMed: 32169822
View all Publications for this product

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