Name: Anti-IRE1 (phospho S724) antibody [EPR5253]
Brand: RabMAb
SKU: ab124945
Rating: 4 (7 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5253] to IRE1 (phospho S724)
Suitable for: WB, Dot blot
Reacts with: Human

Carrier Free

产品名称

Anti-IRE1 (phospho S724)抗体[EPR5253]
参阅全部 IRE1 一抗
描述

兔单克隆抗体[EPR5253] to IRE1 (phospho S724)
宿主

Rabbit
经测试应用

适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF or IHC-P
种属反应性

与反应: Human
免疫原

Synthetic peptide within Human IRE1 aa 700-800 (phospho S724). The exact sequence is proprietary.
Database link: O75460
阳性对照
- WB: Thapsigargin treated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, DTT treated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
解离常数（K_D）

K_D = 1.10 x 10 ^-10 M
Learn more about K_D
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR5253
同种型

IgG
研究领域

Alternative Versions
- Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human IRE1 protein (ab114174)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

The Abpromise guarantee

Abpromise™承诺保证使用ab124945于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(5)	1/200 - 1/10000. Predicted molecular weight: 110 kDa.
Dot blot		1/1000.

说明
WB 1/200 - 1/10000. Predicted molecular weight: 110 kDa.
Dot blot 1/1000.

应用说明

Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

功能

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
组织特异性

Ubiquitously expressed. High levels observed in pancreatic tissue.
序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
翻译后修饰

Autophosphorylated.
细胞定位

Endoplasmic reticulum membrane.
Target information above from: UniProt accession O75460 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- Unigene: 133982 Human
- Unigene: 592041 Human
- Unigene: 700027 Human
别名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
- Endoribonuclease antibody
- ER to nucleus signaling 1 antibody
- ERN 1 antibody
- Ern1 antibody
- ERN1_HUMAN antibody
- hIRE 1p antibody
- hIRE1p antibody
- Inositol requiring 1 antibody
- Inositol requiring 1, S. cerevisiae, homolog of antibody
- Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
- Inositol requiring protein 1 antibody
- inositol-requiring enzyme 1 antibody
- Inositol-requiring protein 1 antibody
- IRE 1 antibody
- IRE 1a antibody
- IRE 1P antibody
- Ire1 alpha antibody
- Ire1-alpha antibody
- IRE1a antibody
- Ire1alpha antibody
- IRE1P antibody
- MGC163277 antibody
- MGC163279 antibody
- Protein kinase/endoribonuclease antibody
- RGD1559716 antibody
- Serine/threonine protein kinase/endoribonuclease IRE1 antibody
see all

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/200 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 1 hour whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 6 hours whole cell lysates
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) treated with 10mM DTT for 1 hour whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 180 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Dot Blot analysis of Lane 1: IRE1 (pS724) phospho peptide and Lane 2: IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab124945？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124945 被引用在 118 文献中.

Jeon MS et al. Polystyrene microplastic particles induce autophagic cell death in BEAS-2B human bronchial epithelial cells. Environ Toxicol 38:359-367 (2023). PubMed: 36485005
Wang Z et al. FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response. Redox Biol 60:102618 (2023). PubMed: 36724577
Qu J et al. Piezo1 suppression reduces demyelination after intracerebral hemorrhage. Neural Regen Res 18:1750-1756 (2023). PubMed: 36751801
Tsuchiya H et al. NEAT1-SOD2 Axis Confers Sorafenib and Lenvatinib Resistance by Activating AKT in Liver Cancer Cell Lines. Curr Issues Mol Biol 45:1073-1085 (2023). PubMed: 36826016
Zhang B et al. MSX1 Regulates Goat Endometrial Function by Altering the Plasma Membrane Transformation of Endometrial Epithelium Cells during Early Pregnancy. Int J Mol Sci 24:N/A (2023). PubMed: 36835532

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-5 of 5 Abreviews or Q&A

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mice hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

45 µg

Specification

Mice hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 05 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (Prostate cancer cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

25 µg

Treatment

100 µM CoCl2

Specification

Prostate cancer cell line

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 15 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing (10 % gel)

Loading amount

30 µg

Treatment

10 ´M tunicamycin for 30 min, 1h, 2h

Specification

HeLa

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tamas Czompoly

Verified customer

提交于 May 19 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293 cells)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

15 µg

Treatment

25 µM Cadmium for 12h

Specification

HEK293 cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 10 2015

Application

Western blot

Sample

Human Cell lysate - other (HeLa cells)

Loading amount

40000 cells

Specification

HeLa cells

Gel Running Conditions

Reduced Denaturing

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 03 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

解离常数（KD）

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (118)

客户评价及客户问答

Western blot abreview for Anti-IRE1 (phospho S724) antibody [EPR5253]

Western blot abreview for Anti-IRE1 (phospho S724) antibody [EPR5253]

Western blot abreview for Anti-IRE1 (phospho S724) antibody [EPR5253]

Western blot abreview for Anti-IRE1 (phospho S724) antibody [EPR5253]

Western blot abreview for Anti-IRE1 (phospho S724) antibody [EPR5253]

解离常数（K_D）