Anti-IRE1抗体(ab37073)
Key features and details
- Rabbit polyclonal to IRE1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-IRE1抗体
参阅全部 IRE1 一抗 -
描述
兔多克隆抗体to IRE1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).
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阳性对照
- WB: Rat brain tissue lysate, A20 and HeLa cell lysates. IHC-P: Rat and Human small intestine tissue. ICC: A20 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).
Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk. Primary ab incubation: 1 h incubation at RT in 5% NFDM/TBST. |
ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa). Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk. Primary ab incubation: 1 h incubation at RT in 5% NFDM/TBST. |
ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
组织特异性
Ubiquitously expressed. High levels observed in pancreatic tissue. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Entrez Gene: 78943 Mouse
- Entrez Gene: 498013 Rat
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- SwissProt: Q9EQY0 Mouse
see all -
别名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
图片
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All lanes : Anti-IRE1 antibody (ab37073) at 0.4 µg/ml
Lane 1 : Caco-2 Cell Lysate
Lane 2 : SK-N-SH Cell Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaDiluting buffer and concentration: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde fixed Human Small Intestine Tissue labeling IRE1 with ab37073 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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All lanes : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
Lane 1 : Wild-type HeLa Cell Lysate
Lane 2 : IRE1 knockout HeLa Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaDiluting buffer and concentration: 1 hour incubation at room temperature in 5% NFDM/TBST.
Beta-actin antibody (1 μg/mL) used as loading control.
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Immunohistochemical analysis of paraffin-embedded Rat Small Intestine Tissue using ab37073 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western Blot of rat brain lysate labeling IRE1 with Anti-IRE1 antibody (ab37073) at (1) 0.5 and (2) 1µg/ml.
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Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted? -
ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.
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Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (153)
ab37073 被引用在 153 文献中.
- Song YF et al. Choline-mediated hepatic lipid homoeostasis in yellow catfish: unravelling choline's lipotropic and methyl donor functions and significance of ire-1α signalling pathway. Br J Nutr 131:202-213 (2024). PubMed: 37642130
- Shi G et al. Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury. J Neuroinflammation 20:222 (2023). PubMed: 37777772
- Yang X et al. Loss of DDRGK1 impairs IRE1α UFMylation in spondyloepiphyseal dysplasia. Int J Biol Sci 19:4709-4725 (2023). PubMed: 37781516
- Meng N et al. PLXNC1 interference alleviates the inflammatory injury, apoptosis and extracellular matrix degradation of IL-1β-exposed chondrocytes via suppressing GRP78 expression. J Orthop Surg Res 18:784 (2023). PubMed: 37853395
- Yu X et al. Apoptin causes apoptosis in HepG-2 cells via Ca2+ imbalance and activation of the mitochondrial apoptotic pathway. Cancer Med 12:8306-8318 (2023). PubMed: 36515089