重组Anti-IRBIT抗体[EPR13409] (ab178693)
![Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.cn/ps/products/178/ab178693/Images/ab178693-206839-anti-irbit-antibody-epr13409-western-blot.jpg "Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13409] to IRBIT
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRBIT抗体[EPR13409]
参阅全部 IRBIT 一抗 -
描述
兔单克隆抗体[EPR13409] to IRBIT -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human IRBIT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O43865 -
阳性对照
- Human thymus, HeLa, Ramos and 293T lysates; HeLa cells; 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13409 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178693于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
|
|
| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
|
ICC/IF
1/250 - 1/500. |
靶标
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组织特异性
Expressed in dendritic cells. -
通路
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
序列相似性
Belongs to the adenosylhomocysteinase family. -
发展阶段
Expression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC. -
结构域
The PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1. -
细胞定位
Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 10768 Human
- Entrez Gene: 229709 Mouse
- Entrez Gene: 362013 Rat
- Omim: 607826 Human
- SwissProt: O43865 Human
- SwissProt: Q80SW1 Mouse
- Unigene: 725986 Human
- Unigene: 220328 Mouse
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别名
- AA409031 antibody
- AdoHcyase 2 antibody
- Ahcy-rs3 antibody
see all
图片
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Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)All lanes : Anti-IRBIT antibody [EPR13409] (ab178693) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : HeLa cell lysate
Lane 3 : Ramos lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.cn/ps/products/178/ab178693/Images/ab178693-206841-anti-irbit-antibody-epr13409-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (ab178693)Immunofluorescent analysis of HeLa cells labeling IRBIT with ab178693 at 1/250 (red) and DAPI staining (blue).
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![Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.cn/ps/products/178/ab178693/Images/ab178693-206842-anti-irbit-antibody-epr13409-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (ab178693)Intracellular flow cytometrical analysis of permeabilized 293T cells labeling IRBIT with ab178693 at 1/10 (red) or a rabbit IgG (negative) (green).
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![Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.cn/ps/products/178/ab178693/Images/ab178693-4-benefits-of-recombinant-antibodies.png)
Anti-IRBIT antibody [EPR13409] (ab178693)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab178693 被引用在 3 文献中.
- Li X et al. AHCYL1 Is a Novel Biomarker for Predicting Prognosis and Immunotherapy Response in Colorectal Cancer. J Oncol 2022:5054324 (2022). PubMed: 35578598
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Kruse M et al. Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. J Neurosci 36:1386-400 (2016). PubMed: 26818524