Anti-IRAKM抗体(ab8116)
Key features and details
- Rabbit polyclonal to IRAKM
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IRAKM抗体
参阅全部 IRAKM 一抗 -
描述
兔多克隆抗体to IRAKM -
宿主
Rabbit -
特异性
Anti-IRAK-M has no cross response to IRAK or IRAK2. -
经测试应用
适用于: ICC, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide:
CSSKFSWDEYEQYKKE
, corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.) -
常规说明
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column. -
Primary antibody说明
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
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| IHC-P |
Use a concentration of 2 µg/ml.
|
|
| WB | (3) |
Use a concentration of 0.5 - 1 µg/ml.
|
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 2 µg/ml. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. -
组织特异性
Expressed predominantly in peripheral blood lymphocytes. -
疾病相关
Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 73914 Mouse
- Entrez Gene: 314870 Rat
- SwissProt: Q8K4B2 Mouse
- Unigene: 146194 Mouse
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别名
- ASRT5 antibody
- IL 1 receptor associated kinase M antibody
- IL-1 receptor-associated kinase M antibody
see all
图片
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Western blot - Anti-IRAKM antibody (ab8116)All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml
Lane 1 : Mouse spleen tissue lysates
Lane 2 : Rat liver tissue lysate
Observed band size: 68 kDa why is the actual band size different from the predicted?
Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1µ -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC
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Immunocytochemistry - Anti-IRAKM antibody (ab8116)Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope. -
Immunocytochemistry - Anti-IRAKM antibody (ab8116)Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.
实验方案
数据表及文件
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Datasheet download
文献 (15)
ab8116 被引用在 15 文献中.
- Sun J et al. Loss of NLRP3 increases bacterial cystitis via IRAKM. Transl Androl Urol 11:268-276 (2022). PubMed: 35280657
- Hu X & Miao H MiR-539-5p inhibits the inflammatory injury in septic H9c2 cells by regulating IRAK3. Mol Biol Rep 49:121-130 (2022). PubMed: 34757596
- Dienel A et al. α7-Acetylcholine Receptor Signaling Reduces Neuroinflammation After Subarachnoid Hemorrhage in Mice. Neurotherapeutics 18:1891-1904 (2021). PubMed: 33970466
- Cai JL et al. Polygonatum sibiricum polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells. Bioengineered 12:10147-10159 (2021). PubMed: 34872451
- Wu X et al. Hypermethylation of the IRAK3-Activated MAPK Signaling Pathway to Promote the Development of Glioma. Cancer Manag Res 12:7043-7059 (2020). PubMed: 32848462
