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AB5985

Anti-IRAK4抗体

Anti-IRAK4 antibody

5

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal IRAK4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK4.

查看别名

Interleukin-1 receptor-associated kinase 4, IRAK-4, Renal carcinoma antigen NY-REN-64, IRAK4

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)
  • ICC/IF

AbReview17009****

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.

Left image : DAPI staining nuclei (blue)
Middle image : IRAK4 (red)
Right image : Overlay

This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview

Western blot - Anti-IRAK4 antibody (AB5985)
  • WB

Supplier Data

Western blot - Anti-IRAK4 antibody (AB5985)

Primary incbation : 1 hour at room temperature in 5% NFDM/TBST.

Lane 1:

Western blot - Anti-IRAK4 antibody (ab5985) at 1 µg/mL

Lane 2:

Western blot - Anti-IRAK4 antibody (ab5985) at 2 µg/mL

Lane 3:

Western blot - Anti-IRAK4 antibody (ab5985) at 4 µg/mL

All lanes:

HeLa cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 52 kDa

false

Western blot - Anti-IRAK4 antibody (AB5985)
  • WB

Supplier Data

Western blot - Anti-IRAK4 antibody (AB5985)

All lanes:

Western blot - Anti-IRAK4 antibody (ab5985) at 1 µg/mL

Lane 1:

Daudi cell lysate at 15 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HepG2 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 52 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human IRAK4. The exact immunogen used to generate this antibody is proprietary information.

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特异性

This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.

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替代产品

Primary Antibodies

AB32511

Anti-IRAK4 antibody [Y279]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [Y279] (AB32511)

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Primary Antibodies

AB216513

Anti-IRAK4 (phospho T345) antibody

Western blot - Anti-IRAK4 (phospho T345) antibody (AB216513)

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Primary Antibodies

AB239819

Anti-IRAK4 antibody [Y279] - BSA and Azide free

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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [Y279] - BSA and Azide free (AB239819)

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Primary Antibodies

AB113525

Anti-IRAK4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody (AB113525)

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Primary Antibodies

AB189108

Anti-IRAK4 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody - C-terminal (AB189108)

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Primary Antibodies

AB119942

Anti-IRAK4 antibody [2H9]

KO Validated

Western blot - Anti-IRAK4 antibody [2H9] (AB119942)

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Primary Antibodies

AB188599

Anti-IRAK4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody (AB188599)

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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
The product can be stored for up to 12 months
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.
Biological function summary

IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.

Pathways

IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.

Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed : 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
See full target information IRAK4
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36040792

2022

Activation of targetable inflammatory immune signaling is seen in myelodysplastic syndromes with SF3B1 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurav S Choudhary,Andrea Pellagatti,Bogos Agianian,Molly A Smith,Tushar D Bhagat,Shanisha Gordon-Mitchell,Srabani Sahu,Sanjay Pandey,Nishi Shah,Srinivas Aluri,Ritesh Aggarwal,Sarah Aminov,Leya Schwartz,Violetta Steeples,Robert N Booher,Murali Ramachandra,Maria Samson,Milagros Carbajal,Kith Pradhan,Teresa V Bowman,Manoj M Pillai,Britta Will,Amittha Wickrema,Aditi Shastri,Robert K Bradley,Robert E Martell,Ulrich G Steidl,Evripidis Gavathiotis,Jacqueline Boultwood,Daniel T Starczynowski,Amit Verma

Mediators of inflammation 2021:5318369 PubMed33505213

2021

lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanchuan Shi,Yuqian Zhao,Qi Li,Jianguo Li

Nature cell biology 21:640-650 PubMed31011167

2019

U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Molly A Smith,Gaurav S Choudhary,Andrea Pellagatti,Kwangmin Choi,Lyndsey C Bolanos,Tushar D Bhagat,Shanisha Gordon-Mitchell,Dagny Von Ahrens,Kith Pradhan,Violetta Steeples,Sanghyun Kim,Ulrich Steidl,Matthew Walter,Iain D C Fraser,Aishwarya Kulkarni,Nathan Salomonis,Kakajan Komurov,Jacqueline Boultwood,Amit Verma,Daniel T Starczynowski

Journal of immunology (Baltimore, Md. : 1950) 179:8491-9 PubMed18056396

2007

Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis.

Applications

WB

Species

Sheep

Suhas G Kallapur,Alan H Jobe,Molly K Ball,Ilias Nitsos,Timothy J M Moss,Noah H Hillman,John P Newnham,Boris W Kramer
View all publications
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