Anti-IRAK2抗体(ab62419)
Key features and details
- Rabbit polyclonal to IRAK2
- Suitable for: ICC/IF, IP, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-IRAK2抗体
参阅全部 IRAK2 一抗 -
描述
兔多克隆抗体to IRAK2 -
宿主
Rabbit -
特异性
This antibody reacts with IRAK2, it does not react with IRAK -
经测试应用
适用于: ICC/IF, IP, ICC, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).
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阳性对照
- WB: RAW264.7 whole cell lysate. ICC: A-20 cells. ICC/IF: A-20 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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|
| IP | (1) |
Use at an assay dependent concentration.
(PMID 19224918) |
| ICC |
Use a concentration of 1 µg/ml.
|
|
| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
IP
Use at an assay dependent concentration. (PMID 19224918) |
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ICC
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. -
组织特异性
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. -
结构域
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
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数据库链接
- Entrez Gene: 3656 Human
- Entrez Gene: 108960 Mouse
- Omim: 603304 Human
- SwissProt: O43187 Human
- SwissProt: Q8CFA1 Mouse
- Unigene: 449207 Human
- Unigene: 152142 Mouse
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别名
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
see all
图片
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Western blot - Anti-IRAK2 antibody (ab62419)This image is courtesy of an anonymous abreview.
Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
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Western blot - Anti-IRAK2 antibody (ab62419)Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml
Lane 1 : RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lanes 2-3 : RAW264.7 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry - Anti-IRAK2 antibody (ab62419)Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab62419)Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab62419 被引用在 13 文献中.
- Liu W et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun 13:2748 (2022). PubMed: 35585086
- McCrory EH et al. Identification of ester-linked ubiquitylation sites during TLR7 signalling increases the number of inter-ubiquitin linkages from 8 to 12. Biochem J 479:2419-2431 (2022). PubMed: 36408944
- Xiao T et al. lncRNA NEAT1 mediates sepsis progression by regulating Irak2 via sponging miR-370-3p. Biol Open 9:N/A (2020). PubMed: 32414769
- Zhou H et al. IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes. Nat Immunol 21:1219-1231 (2020). PubMed: 32778760
- Zhou H et al. IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs. Elife 6:N/A (2017). PubMed: 28990926