Anti-IRAK-1抗体(ab238)
Key features and details
- Rabbit polyclonal to IRAK-1
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-IRAK-1抗体
参阅全部 IRAK-1 一抗 -
描述
兔多克隆抗体to IRAK-1 -
宿主
Rabbit -
特异性
At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
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经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human IRAK-1 aa 700-712 (C terminal).
(Peptide available asab6230) -
阳性对照
- WB: L1210 and YB2/0 cell lysates (Human, Mouse & Rat). ICC: HeLa and murine RAW 264.7 cells (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000. Predicted molecular weight: 77 kDa.
(THP-1 or HeLa whole cell) from non-activated cells. |
ICC |
Use a concentration of 10 µg/ml.
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说明 |
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WB
1/1000. Predicted molecular weight: 77 kDa. (THP-1 or HeLa whole cell) from non-activated cells. |
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
组织特异性
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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数据库链接
- Entrez Gene: 3654 Human
- Entrez Gene: 16179 Mouse
- Entrez Gene: 363520 Rat
- Omim: 300283 Human
- SwissProt: P51617 Human
- SwissProt: Q62406 Mouse
- Unigene: 522819 Human
- Unigene: 38241 Mouse
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别名
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
see all
图片
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All lanes : Anti-IRAK-1 antibody (ab238) at 1 µg/ml
Lane 1 : L1210 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa1h incubation at RT in 5% NFDM/TBST.
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ab238 at 10µg/ml staining Hela cells by ICC.
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Immunocytochemistry - Anti-IRAK-1 antibody (ab238)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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ICC of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab238 被引用在 29 文献中.
- Bokobza C et al. miR-146b Protects the Perinatal Brain against Microglia-Induced Hypomyelination. Ann Neurol 91:48-65 (2022). PubMed: 34741343
- Gratal P et al. 6-Shogaol (enexasogoal) treatment improves experimental knee osteoarthritis exerting a pleiotropic effect over immune innate signalling responses in chondrocytes. Br J Pharmacol 179:5089-5108 (2022). PubMed: 35760458
- Chen W et al. IL-1 receptor-associated kinase 1 participates in the modulation of the NLRP3 inflammasome by tumor-associated macrophages in hepatocellular carcinoma. J Gastrointest Oncol 13:1317-1329 (2022). PubMed: 35837195
- Zhao G et al. Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics. Front Microbiol 13:989183 (2022). PubMed: 36171749
- Ji L et al. Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4+ T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling. Evid Based Complement Alternat Med 2022:5161890 (2022). PubMed: 36467552