Anti-IRAK-1抗体(ab238)
Key features and details
- Rabbit polyclonal to IRAK-1
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-IRAK-1抗体
参阅全部 IRAK-1 一抗 -
描述
兔多克隆抗体to IRAK-1 -
宿主
Rabbit -
特异性
At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
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经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human IRAK-1 aa 700-712 (C terminal).
(Peptide available asab6230) -
阳性对照
- WB: L1210 and YB2/0 cell lysates (Human, Mouse & Rat). ICC: HeLa and murine RAW 264.7 cells (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/1000. Predicted molecular weight: 77 kDa.
(THP-1 or HeLa whole cell) from non-activated cells. |
| ICC |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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WB
1/1000. Predicted molecular weight: 77 kDa. (THP-1 or HeLa whole cell) from non-activated cells. |
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ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
组织特异性
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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数据库链接
- Entrez Gene: 3654 Human
- Entrez Gene: 16179 Mouse
- Entrez Gene: 363520 Rat
- Omim: 300283 Human
- SwissProt: P51617 Human
- SwissProt: Q62406 Mouse
- Unigene: 522819 Human
- Unigene: 38241 Mouse
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别名
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
see all
图片
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Western blot - Anti-IRAK-1 antibody (ab238)All lanes : Anti-IRAK-1 antibody (ab238) at 1 µg/ml
Lane 1 : L1210 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa1h incubation at RT in 5% NFDM/TBST.
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Immunocytochemistry - Anti-IRAK-1 antibody (ab238)ab238 at 10µg/ml staining Hela cells by ICC.
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Immunocytochemistry - Anti-IRAK-1 antibody (ab238)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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Immunocytochemistry - Anti-IRAK-1 antibody (ab238)ICC of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab238 被引用在 38 文献中.
- Song J et al. Bifidobacterium mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway. Histol Histopathol 39:623-632 (2024). PubMed: 37916940
- Jung SH et al. Ethanol Extracts from the Aerial Parts of Inula japonica and Potentilla chinensis Alleviate Airway Inflammation in Mice That Inhaled Particulate Matter 10 and Diesel Particulate Matter. Nutrients 15:N/A (2023). PubMed: 37960252
- Huang J et al. Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway. Phytother Res 37:731-742 (2023). PubMed: 36196887
- Jin J et al. Effects of acupuncture on the miR-146a-mediated IRAK1/TRAF6/NF-κB signaling pathway in rats with sarcopenia induced by D-galactose. Ann Transl Med 11:47 (2023). PubMed: 36819511
- Liang XS et al. IRAK-M Ablation Promotes Status Epilepticus-Induced Neuroinflammation via Activating M1 Microglia and Impairing Excitatory Synaptic Function. Mol Neurobiol 60:5199-5213 (2023). PubMed: 37277682