AB238

Anti-IRAK-1抗体

Name: IRAK-1抗体 (ab238)| Abcam中文官网
Brand: Abcam Limited
SKU: AB238
Price: 5596 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-IRAK-1 antibody

(6 Reviews)

(42 Publications)

Rabbit Polyclonal IRAK-1 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK1 aa 700 to C-terminus.

查看别名

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1

4 Images

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Immunocytochemistry - Anti-IRAK-1 antibody (AB238)

ab238 at 10μg/ml staining Hela cells by ICC.

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Immunocytochemistry - Anti-IRAK-1 antibody (AB238)

ICC of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.

AbReview30355****

Immunocytochemistry - Anti-IRAK-1 antibody (AB238)

ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.

Image courtesy of Dr Mahesh Shivananjappa by Abreview.

Supplier Data

Western blot - Anti-IRAK-1 antibody (AB238)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRAK-1 antibody (ab238) at 1 µg/mL

Lane 1:

L1210 cell lysate at 15 µg

Lane 2:

YB2/0 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 76 kDa

Observed band size: 77 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC, WB

applications

免疫原

Synthetic Peptide within Human IRAK1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P51617

特异性

At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(THP-1 or HeLa whole cell) from non-activated cells." }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(THP-1 or HeLa whole cell) from non-activated cells." } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Affinity chromatography purified via peptide column.

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.

Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

See full target information IRAK1

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文献 (42)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 22:3556-3564 PubMed40959576

2025

Vascular Endothelial Protection Effects of Root Aqueous Extracts on LPS-induced Rat Vascular Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hsien Chen,Sheau-Chung Tang,Shu-Er Yang,Erl-Shyh Kao

Investigative and clinical urology 65:84-93 PubMed38197755

2024

IFITM3-mediated activation of TRAF6/MAPK/AP-1 pathways induces acquired TKI resistance in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Se Un Jeong,Ja-Min Park,Sun Young Yoon,Hee Sang Hwang,Heounjeong Go,Dong-Myung Shin,Hyein Ju,Chang Ohk Sung,Jae-Lyun Lee,Gowun Jeong,Yong Mee Cho

Histology and histopathology 39:623-632 PubMed37916940

2023

mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen Zhang

Nutrients 15: PubMed37960252

2023

Ethanol Extracts from the Aerial Parts of and Alleviate Airway Inflammation in Mice That Inhaled Particulate Matter 10 and Diesel Particulate Matter.

Applications

Unspecified application

Species

Unspecified reactive species

Seang-Hwan Jung,Kyung-Sook Chung,Chang-Seon Na,Hye-Shin Ahn,Yu-Kyong Shin,Kyung-Tae Lee

Scientific reports 13:11192 PubMed37433841

2023

MicroRNA-146a negatively regulates inflammation via the IRAK1/TRAF6/NF-κB signaling pathway in dry eye.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifang Han,Juan Gao,Liming Wang,Peng Hao,Xi Chen,Yuchuan Wang,Zhixin Jiang,Li Jiang,Ting Wang,Lin Zhu,Xuan Li

Molecular neurobiology 60:5199-5213 PubMed37277682

2023

IRAK-M Ablation Promotes Status Epilepticus-Induced Neuroinflammation via Activating M1 Microglia and Impairing Excitatory Synaptic Function.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Shan Liang,Ting-Lin Qian,Yi-Fan Xiong,Xiao-Tao Liang,Yue-Wen Ding,Xiao-Yu Zhu,Yun-Lv Li,Jie-Li Zhou,Le-Yi Tan,Wei-Peng Li,Wei Xie

Annals of translational medicine 11:47 PubMed36819511

2023

Effects of acupuncture on the miR-146a-mediated IRAK1/TRAF6/NF-κB signaling pathway in rats with sarcopenia induced by D-galactose.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Jin,Zhengyu Yang,Haichao Liu,Mingling Guo,Borui Chen,Haoming Zhu,Yu Wang,Jianping Lin,Shizhong Wang,Shaoqing Chen

Evidence-based complementary and alternative medicine : eCAM 2022:5161890 PubMed36467552

2022

Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4 T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Ji,Shenglong Wang,Shan Wu,Jie Bao,Guanqun Xie,Yan Zhang,Liping Xu,Na Lin,Jian Wang,Yongsheng Fan,Danqing Fu,Qiaoding Dai

Phytotherapy research : PTR 37:731-742 PubMed36196887

2022

Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Huang,Tiantian Wu,Youbao Zhong,Jiaqi Huang,Zengping Kang,Bugao Zhou,Haimei Zhao,Duanyong Liu

Frontiers in microbiology 13:989183 PubMed36171749

2022

Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Guanyu Zhao,Yan Gao,Jiaqi Zhang,He Zhang,Changzhan Xie,Fulong Nan,Sheng Feng,Zhuo Ha,Chenghui Li,Xiangyu Zhu,Zhuoxin Li,Ping Zhang,Ying Zhang,Huijun Lu,Ningyi Jin

View all publications

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