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使用敲除细胞株进行验证重组RabMAb

重组Anti-IQGAP1抗体[EPR5220] - BSA and Azide free (ab271893)

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Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
  • Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
  • Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5220] to IQGAP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

概述

  • 产品名称

    Anti-IQGAP1抗体[EPR5220] - BSA and Azide free
    参阅全部 IQGAP1 一抗

  • 描述

    兔单克隆抗体[EPR5220] to IQGAP1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IHC-P: Human kidney tissue

  • 常规说明

    ab271893 is the carrier-free version of ab133490.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271893于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 189 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 189 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.

  • 组织特异性

    Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

  • 序列相似性

    Contains 1 CH (calponin-homology) domain.
    Contains 4 IQ domains.
    Contains 1 Ras-GAP domain.
    Contains 1 WW domain.

  • 结构域

    Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.

  • 翻译后修饰

    Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • HUMORFA01 antibody
      • IQ motif containing GTPase activating protein 1 antibody
      • IQGA1_HUMAN antibody
      • Iqgap1 antibody
      • KIAA0051 antibody
      • p195 antibody
      • Ras GTPase activating like protein 1 antibody
      • Ras GTPase-activating-like protein IQGAP1 antibody
      • RasGAP-like with IQ motifs antibody
      • SAR1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
      Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IQGAP1 with Purified ab133490 at 1/500 dilution (5 µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133490).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)

      Immunohistochemical analysis of IQGAP1 in paraffin embedded Human kidney tissue, using ab133490 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133490).
    • Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
      Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IQGAP1 with unpurified ab133490 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133490). 

       

       

    • Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)
      Anti-IQGAP1 antibody [EPR5220] - BSA and Azide free (ab271893)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271893？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271893 尚未被引用在任何文献中。

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