Anti-IP3 receptor抗体(ab97823)
Key features and details
- Rabbit polyclonal to IP3 receptor
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IP3 receptor抗体
参阅全部 IP3 receptor 一抗 -
描述
兔多克隆抗体to IP3 receptor -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human IP3 receptor aa 2700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab107795) -
阳性对照
- This antibody gave a positive signal in HeLa and PC3 Whole Cell Lysates. A band of the expected size was also detected in MALME 3M and Raji Whole Cell Lysates (data not shown). In our hands, the signal was relatively weak in these lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa). |
靶标
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功能
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. -
组织特异性
Widely expressed. -
疾病相关
Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. -
序列相似性
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
结构域
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
翻译后修饰
Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3708 Human
- Entrez Gene: 16438 Mouse
- Entrez Gene: 25262 Rat
- Omim: 147265 Human
- SwissProt: Q14643 Human
- SwissProt: P11881 Mouse
- SwissProt: P29994 Rat
- Unigene: 567295 Human
see all -
别名
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IP3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY-5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab97823 observed at 314 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab97823 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when IP3 knockout cells were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. Ab97823 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-IP3 receptor antibody (ab97823) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 314 kDa
Observed band size: 314 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97823 被引用在 1 文献中.
- Peggion C et al. Regulation of Endoplasmic Reticulum-Mitochondria Tethering and Ca2+ Fluxes by TDP-43 via GSK3β. Int J Mol Sci 22:N/A (2021). PubMed: 34769284