重组Anti-Ionotropic Glutamate receptor 2抗体[EPR5032] (ab133477)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5032] to Ionotropic Glutamate receptor 2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Ionotropic Glutamate receptor 2抗体[EPR5032]
参阅全部 Ionotropic Glutamate receptor 2 一抗 -
描述
兔单克隆抗体[EPR5032] to Ionotropic Glutamate receptor 2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 150-250. The exact sequence is proprietary.
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阳性对照
- Flow Cyt (intra): SH-SY5Y cells; ICC/IF: Neuro-2a cells WB: Human hypothalamus and midbrain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5032 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133477于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/80.
|
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
|
ICC/IF |
1/70.
|
说明 |
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Flow Cyt (Intra)
1/80. |
WB
1/1000 - 1/10000. Predicted molecular weight: 98 kDa. |
ICC/IF
1/70. |
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
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数据库链接
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
别名
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
图片
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All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/5000 dilution (Purified)
Lane 1 : Human hypothalamus lysate
Lane 2 : Human midbrain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 98 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Ionotropic Glutamate receptor 2 with purified ab133477 at 1/70 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab133477 被引用在 31 文献中.
- Hassan Z et al. Cognitive Impairment Is Associated with AMPAR Glutamatergic Dysfunction in a Mouse Model of Neuronal Methionine Synthase Deficiency. Cells 12:N/A (2023). PubMed: 37174668
- Ge J et al. MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice. JCI Insight 8:N/A (2023). PubMed: 37192007
- González-Alfonso WL et al. Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspring. Arch Toxicol 97:2371-2383 (2023). PubMed: 37482551
- Chen Y et al. Inhibiting NLRP3 inflammasome signaling pathway promotes neurological recovery following hypoxic-ischemic brain damage by increasing p97-mediated surface GluA1-containing AMPA receptors. J Transl Med 21:567 (2023). PubMed: 37620837
- Zhang Y & Jeske NA A-kinase anchoring protein 79/150 coordinates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor sensitization in sensory neurons. Mol Pain 19:17448069231222406 (2023). PubMed: 38073552